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Multiple Myeloma

Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Ross D. Brown

    1. Institute of Haematology, Royal Prince Alfred Hospital, Sydney, Australia

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    You can also search for this editor in PubMed   Google Scholar

  2. P. Joy Ho

    1. Institute of Haematology, Royal Prince Alfred Hospital, Sydney, Australia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Medicine (MIMM, volume 113)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Multiple Myeloma

    • Douglas Joshua, Ross Brown, P. Joy Ho
    Pages 1-4

  3. Immunophenotyping of Plasma Cells in Multiple Myeloma

    • Gema Mateo Manzanera, Jesús F. San Miguel Izquierdo, Alberto Orfao de Matos
    Pages 5-24

  4. Plasma Cell Labeling Index

    • Philip R. Greipp, Shaji Kumar
    Pages 25-35

  5. Conventional Cytogenetics in Myeloma

    • Lynda J. Campbell
    Pages 37-48

  6. Multicolor Spectral Karyotyping in Multiple Myeloma

    • Jeffrey R. Sawyer
    Pages 49-58

  7. Detection of Chromosome 13 Deletions by Fluorescent In Situ Hybridization

    • Scott VanWier, Rafael Fonseca
    Pages 59-69

  8. Comparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple Myeloma

    • Pulivarthi H. Rao
    Pages 71-83

  9. Southern Blotting of IgH Rearrangements in B-Cell Disorders

    • Edna Yuen, Ross D. Brown
    Pages 85-103

  10. Identification and Assembly of V Genes as Idiotype-Specific DNA Fusion Vaccines in Multiple Myeloma

    • Surinder S. Sahota, Mark Townsend, Freda K. Stevenson
    Pages 105-119

  11. Identification of Clonotypic IgH VDJ Sequences in Multiple Myeloma

    • Brian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, Linda M. Pilarski
    Pages 121-144

  12. Real-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in Myeloma

    • Mara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, Marco Ladetto
    Pages 145-163

  13. Incomplete DJH Rearrangements

    • David González, Ramón Garcia-Sanz
    Pages 165-173

  14. Identification of Malignant Plasma Cells by mRNA In Situ Hybridization

    • Ross D. Brown, P. Joy Ho
    Pages 175-181

  15. The SCID-hu Myeloma Model

    • Joshua Epstein, Shmuel Yaccoby
    Pages 183-190

  16. The 5T2MM Murine Model of Multiple Myeloma

    • Karin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik de Raeve, Pedro Couck, Frans Gorus et al.
    Pages 191-205

  17. Determination of Telomerase Activity and Telomere Length

    • Kai-da Wu, Malcolm A. S. Moore
    Pages 207-223

  18. Dendritic Cell/Myeloma Hybrid Vaccine

    • Dajing Xia, Tim Chan, Jim Xiang
    Pages 225-234

  19. Genetically Engineered Myeloma Cell Vaccine

    • Siguo Hao, Tim Chan, Jim Xiang
    Pages 235-244

  20. An In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating Factors

    • Andrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, Gerald J. Atkins
    Pages 245-256
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Keywords

About this book

A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.

Reviews

From the reviews:

"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists

"Audience: Hematologists, oncologists, and other clinicians … as well as biomedical researchers in the field of hematology and immunology. … The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. … covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics.” (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)

Editors and Affiliations

  • Institute of Haematology, Royal Prince Alfred Hospital, Sydney, Australia

    Ross D. Brown, P. Joy Ho

Bibliographic Information

  • Book Title: Multiple Myeloma

  • Book Subtitle: Methods and Protocols

  • Editors: Ross D. Brown, P. Joy Ho

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599168

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-392-3Published: 01 April 2005

  • Softcover ISBN: 978-1-61737-537-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-916-5Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 310

  • Number of Illustrations: 69 b/w illustrations, 1 illustrations in colour

  • Topics: Hematology

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