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Nuclear Reprogramming

Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Steve Pells

    1. Department of Gene Function and Development, Roslin Institute, Roslin

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 325)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Nuclear Transplantation in Xenopus

    • John B. Gurdon
    Pages 1-9

  3. Nuclear Transfer in Sheep

    • William A. Ritchie
    Pages 11-23

  4. Protocols for Nuclear Transfer in Mice

    • Shaorong Gao
    Pages 25-33

  5. Isolation of Stromal Stem Cells From Human Adipose Tissue

    • Andrew C. Boquest, Aboulghassem Shahdadfar, Jan E. Brinchmann, Philippe Collas
    Pages 35-46

  6. Nuclear Reprogramming by Cell Fusion

    • Robert H. Broyles, Austin C. Roth, Mairead Todd, Visar Belegu
    Pages 47-57

  7. Polyethylene Glycol-Mediated Cell Fusion

    • Jian Yang, Ming Hong Shen
    Pages 59-66

  8. Epigenetic Reprogramming of Somatic Genomes by Electrofusion With Embryonic Stem Cells

    • Masako Tada, Takashi Tada
    Pages 67-79

  9. Quantification of Cell Fusion by Flow Cytometry

    • Stephen Sullivan, Martin Waterfall, Ed J. Gallagher, Jim McWhir, Steve Pells
    Pages 81-97

  10. Modulation of Cell Fate Using Nuclear and Cytoplasmic Extracts

    • Anne-Mari Håkelien, Kristine G. Gaustad, Philippe Collas
    Pages 99-114

  11. Transgenic Systems in Nuclear Reprogramming

    • Megan Munsie, Peter Mountford, Jennifer Nichols
    Pages 115-128

  12. Using Immunofluorescence to Observe Methylation Changes in Mammalian Preimplantation Embryos

    • Fátima Santos, Wendy Dean
    Pages 129-138

  13. Observing S-Phase Dynamics of Histone Modifications With Fluorescently Labeled Antibodies

    • Rong Wu, Anna V. Terry, David M. Gilbert
    Pages 139-148

  14. Quantitative Analysis of Telomerase Activity and Telomere Length in Domestic Animal Clones

    • Dean H. Betts, Steven Perrault, Lea Harrington, W. Allan King
    Pages 149-180

  15. Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells

    • Cornelia Wiese, Gabriela Kania, Alexandra Rolletschek, Przemyslaw Blyszczuk, Anna M. Wobus
    Pages 181-205

  16. Staining Embryonic Stem Cells Using Monoclonal Antibodies to Stage-Specific Embryonic Antigens

    • Bruce A. Fenderson, Maria P. De Miguel, April D. Pyle, Peter J. Donovan
    Pages 207-224

  17. Analysis of the Nucleolar Compartment of the Nucleus as an Indicator of Nuclear Reprogramming After Nuclear Transfer

    • Jacques-E. Fléchon
    Pages 225-238

  18. Methylation-Sensitive Polymerase Chain Reaction

    • Hannah R. Moore, Richard R. Meehan, Lorraine E. Young
    Pages 239-249

  19. Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis

    • Yong-Mahn Han, Seok-Ho Kim, Yong-Kook Kang
    Pages 251-260

  20. Chromatin Immunoprecipitation Assay for Mammalian Tissues

    • Fiona B. Turner, Wang L. Cheung, Peter Cheung
    Pages 261-272
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Keywords

About this book

A wide-ranging collection of readily reproducible methods for performing nuclear reprogramming by nuclear transfer in several different species, by fusion through both chemical treatment and electrically shocking cells, and by in vivo treatment of cells with cell extracts. Several methods of monitoring nuclear reprogramming are also presented, including the use of transgenic markers, activation of telomerase as an ES-specific marker, light and electron microscopic observation of structural changes in the nucleus, and verification of surface marker expression and the differentiation potential of stem cells. Biochemical methods are provided for the examination of chromatin protein modifications, nucleosomal footprinting, transcription factor binding, and the study of DNA methylation changes both at the specific locus level and at the level of the whole nucleus.

Editors and Affiliations

  • Department of Gene Function and Development, Roslin Institute, Roslin

    Steve Pells

Bibliographic Information

  • Book Title: Nuclear Reprogramming

  • Book Subtitle: Methods and Protocols

  • Editors: Steve Pells

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1597450057

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-379-4Published: 05 December 2005

  • Softcover ISBN: 978-1-61737-530-9Published: 20 December 2010

  • eBook ISBN: 978-1-59745-005-8Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 334

  • Topics: Biochemistry, general, Cell Biology

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