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Clinical Applications of PCR

  • Book
  • © 2006

Overview

Editors:
  1. Y. M. Dennis Lo

    1. Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China

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  2. Rossa W. K. Chiu

    1. Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China

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    You can also search for this editor in PubMed   Google Scholar

  3. K. C. Allen Chan

    1. Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 336)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. Introduction to the Polymerase Chain Reaction

    • Y. M. Dennis Lo, K. C. Allen Chan
    Pages 1-10

  3. Setting Up a Polymerase Chain Reaction Laboratory

    • Y. M. Dennis Lo, K. C. Allen Chan
    Pages 11-18

  4. Real-Time Polymerase Chain Reaction and Melting Curve Analysis

    • Robert J. Pryor, Carl T. Wittwer
    Pages 19-32

  5. Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses

    • Ivy H. N. Wong
    Pages 33-43

  6. In-Cell Polymerase Chain Reaction

    • T. Vauvert Hviid
    Pages 45-58

  7. Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

    • Chunming Ding
    Pages 59-71

  8. Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography

    • Ching-Wan Lam
    Pages 73-82

  9. Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies

    • Bernhard Zimmermann, Lisa Levett, Wolfgang Holzgreve, Sinuhe Hahn
    Pages 83-100

  10. Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma

    • Rossa W. K. Chiu, Y. M. Dennis Lo
    Pages 101-109

  11. Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA

    • K. C. Allen Chan, Y. M. Dennis Lo
    Pages 111-121

  12. Molecular Analysis of Circulating RNA in Plasma

    • Nancy B. Y. Tsui, Ng Enders K. O., Y. M. Dennis Lo
    Pages 123-134

  13. Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction

    • Lee-JunC. Wong, Bryan R. Cobb, Tian-Jian Chen
    Pages 135-143

  14. Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis

    • Anatoly V. Lichtenstein, Hovsep S. Melkonyan, L. David Tomei, Samuil R. Umansky
    Pages 145-154

  15. Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction

    • Shira Gal, James S. Wainscoat
    Pages 155-162

  16. Molecular Diagnosis of Severe Acute Respiratory Syndrome

    • Enders K. O. Ng, Y. M. Dennis Lo
    Pages 163-175

  17. Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus

    • Stephen S. C. Chim, Rossa W. K. Chiu, Y. M. Dennis Lo
    Pages 177-194

  18. Back Matter

    Pages 195-200
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Keywords

About this book

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

Reviews

From the reviews:

"...will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists." -Archives of Pathology and Laboratory Medicine

"Each chapter is clearly written and provides enough background material to explain the significance and application of the procedure. The protocols include sufficient detail to enable the reader to develop the test. This manual will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists."-Archives of Pathology and Laboratory Medicine

"...a unique document that will be welcome in the clinical laboratory as well as the research field." -Doody's Health Sciences Book Review Journal

"This book is pitched at both pathologists and microbiologists who use PCR in a diagnostic setting. It provides a user-friendly and relatively brief theoretical background to conventional and real-time PCR … . This book should appeal to professional laboratory scientists and students … as well as to more experienced individuals who want to use or extend specific applications. This book would be a useful purchase as a reference source and technical manual for diagnostic pathology and microbiology laboratories." (David Hampson, SGM- Society for General Microbiology, 2007)

Editors and Affiliations

  • Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China

    Y. M. Dennis Lo, Rossa W. K. Chiu, K. C. Allen Chan

Bibliographic Information

  • Book Title: Clinical Applications of PCR

  • Editors: Y. M. Dennis Lo, Rossa W. K. Chiu, K. C. Allen Chan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/159745074X

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-348-0Published: 01 March 2006

  • Softcover ISBN: 978-1-61737-511-8Published: 01 December 2010

  • eBook ISBN: 978-1-59745-074-4Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 200

  • Number of Illustrations: 47 b/w illustrations

  • Topics: Cell Biology

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