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Differential Display Methods and Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Peng Liang

    1. Department of Cancer Biology, The Vanderbilt-Ingram Cancer Center, Nashville

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  2. Jonathan D. Meade

    1. GenHunter Corporation, Nashville

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    You can also search for this editor in PubMed   Google Scholar

  3. Arthur B. Pardee

    1. Dana Farber Cancer Institute, Boston

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Part of the book series: Methods in Molecular Biology (MIMB, volume 317)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Methodologies

    1. Front Matter

      Pages 1-1
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    2. Global Analysis of Gene Expression by Differential Display

      • Shitao Yang, Peng Liang
      Pages 3-21

    3. Automation of Fluorescent Differential Display With Digital Readout

      • Jonathan D. Meade, Yong-jig Cho, Jeffrey S. Fisher, Jamie C. Walden, Zhen Guo, Peng Liang
      Pages 23-57

    4. Ordered Differential Display

      • Mikhail V. Matz, Ella A. Meleshkevitch
      Pages 59-74

    5. GeneCalling®

      • Richard A. Shimkets
      Pages 75-83

    6. High-Density Sampling Differential Display of Prokaryotic mRNAs With RAP-PCR

      • Dana M. Walters, Pierre E. Rouvière
      Pages 85-97

    7. Vertical Arrays

      • Rosana Risques, Gaelle Rondeau, Martin Judex, Michael McClelland, John Welsh
      Pages 99-109

    8. Automated Pattern Ranking in Differential Display Data Analysis

      • Tero Aittokallio, Pekka Ojala, Timo J. Nevalainen, Olli S. Nevalainen
      Pages 111-122

    9. Linking cDNA-AFLP-Based Gene Expression Patterns and ESTs

      • Ling Qin, Pjotr Prins, Johannes Helder
      Pages 123-138

  3. Applications

    1. Front Matter

      Pages 139-139
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    2. Differentially Expressed Genes Associated With Hepatitis B Virus HBx and MHBs Protein Function in Hepatocellular Carcinoma

      • Dae-Ghon Kim
      Pages 141-155

    3. Identification of Disease Markers by Differential Display

      • Michael Clinton, Gino Miele, Sunil Nandi, Derek McBride
      Pages 157-178

    4. Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display

      • Yong-jig Cho, Susanne Stein, Roger S. Jackson, Peng Liang
      Pages 179-192

    5. Identification of p53-Regulated Genes by the Method of Differential Display

      • Yunping Lin, Roger P. Leng, Samuel Benchimol
      Pages 193-206

    6. Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes

      • Zhongjia Tan, Mai Wang, Peng Liang
      Pages 207-218

    7. Comprehensive Analysis of Ovarian Gene Expression During Ovulation Using Differential Display

      • Lawrence L. Espey
      Pages 219-241

    8. Functional Analysis of Nocturnin

      • Julie E. Baggs, Carla B. Green
      Pages 243-254

    9. Isolation and Characterization of Anthocyanin 5-O-Glucosyltransferase in Perilla frutescens var. crispa by Differential Display

      • Mami Yamazaki, Kazuki Saito
      Pages 255-266

    10. Identification of Target Genes of a Yeast Transcriptional Repressor

      • Bernard Mai, Linda L. Breeden
      Pages 267-277
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Keywords

About this book

Since the first edition of this book dedicated to differential display (DD) technology was published in 1997, we have witnessed an explosive interest in studying differential gene expression. The gene-hunting euphoria was initially powered by the invention of DD, which was gradually overtaken by DNA microarray technology in recent years. Then why is there still the need for second edition of this DD book? First of all, DD still enjoys a substantial lead over DNA microarrays in the ISI citation data (see Table 1), despite the h- dreds of millions of dollars spent each year on arrays. This may come as a surprise to many, but to us it implies that many of the DNA microarray studies went unpublished owing to their unfulfilled promises (1). Second, unlike DNA microarrays, DD is an “open”-ended gene discovery method that does not depend on prior genome sequence information of the organism being studied. As such, DD is applicable to the study of all living organisms—from bacteria, fungi, insects, fish, plants, to mammals—even when their genomes are not sequenced. Second, DD is more accessible technically and financially to most cost-conscious “cottage-industry” academic laboratories. So clearly DD still has its unique place in the modern molecular biological toolbox for gene expression analysis.

Reviews

"The variety of applications...is so broad that almost every newcomer...can use these protocols as a starting point for a specific study...highly recommended for purchase by everyone interested in the comparative gene expression, and especially to newcomers to the field." -Trends in Biotechnology

From Reviews of the First Edition...
"...really helpful for all geneticists and cellular and molecular biologists...a precious book which should be in all hands!" -Cellular and Molecular Biology

"...For any would-be gene hunter thinking of or already practicing differential display, this book will provide an invaluable and long overdue guide to the technique." -Molecular Medicine

"....an ideal resource for any research group interested in using DD." -Microbiology Today

"...highly recommend[ed]...for purchase by everyone interested in the comparative gene expression,, and especially to newcomers to the field." -Trends in Biotechnology

Editors and Affiliations

  • Department of Cancer Biology, The Vanderbilt-Ingram Cancer Center, Nashville

    Peng Liang

  • GenHunter Corporation, Nashville

    Jonathan D. Meade

  • Dana Farber Cancer Institute, Boston

    Arthur B. Pardee

Bibliographic Information

  • Book Title: Differential Display Methods and Protocols

  • Editors: Peng Liang, Jonathan D. Meade, Arthur B. Pardee

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599680

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-338-1Published: 21 October 2005

  • Softcover ISBN: 978-1-61737-503-3Published: 09 December 2010

  • eBook ISBN: 978-1-59259-968-4Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 320

  • Number of Illustrations: 131 b/w illustrations, 1 illustrations in colour

  • Topics: Cell Biology

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