Peptide Synthesis and Applications

1. Front Matter

   Pages i-x

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2. Common Strategies

   1. Front Matter

      Pages 1-1

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   2. Fundamentals of Modern Peptide Synthesis

      • Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, Gilles Subra

      Pages 3-24

   3. Chimerism

      • John Howl

      Pages 25-41

3. Synthetic Methodologies and Applications

   1. Front Matter

      Pages 43-43

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   2. Modification of Peptides and Other Drugs Using Lipoamino Acids and Sugars

      • Joanne T. Blanchfield, Istvan Toth

      Pages 45-61

   3. Synthesis of Linear, Branched, and Cyclic Peptide Chimera

      • Gábor Mezö, Ferenc Hudecz

      Pages 63-76

   4. Synthesis of Cell-Penetrating Peptides for Cargo Delivery

      • Margus Pooga, lo Langel

      Pages 77-89

   5. Incorporation of Phosphotyrosyl Mimetic 4- (Phosphonodifluoromethyl)phenylalanine (F2Pmp) Into Signal Transduction-Directed Peptides

      • Zhu-Jun Yao, Kyeong Lee, Terrence R. Burke Jr.

      Pages 91-103

   6. Expressed Protein Ligation for Protein Semisynthesis and Engineering

      • Zuzana Machova, Annette G. Beck-Sickinger

      Pages 105-130

   7. Cellular Delivery of Peptide Nucleic Acid by Cell-Penetrating Peptides

      • Kalle Kilk, lo Langel

      Pages 131-141

   8. Quenched Fluorescent Substrate- Based Peptidase Assays

      • Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, A. Ian Smith

      Pages 143-150

   9. A Convenient Method for Synthesis of Cyclic Peptide Libraries

      • Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton et al.

      Pages 151-166

   10. High-Throughput Peptide Synthesis

       • Michal Lebl, John Hachmann

       Pages 167-194

   11. Backbone Amide Linker Strategies for the Solid-Phase Synthesis of C - Terminal Modified Peptides

       • Jordi Alsina, Steven A. Kates, George Barany, Fernando Albericio

       Pages 195-208

   12. 13 Synthesis of Peptide Bioconjugates

       • Ferenc Hudecz

       Pages 209-223

4. Practical Guides

   1. Front Matter

      Pages 225-225

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   2. Protein Identification by Mass Spectrometric Analyses of Peptides

      • Ashley Martin

      Pages 227-240

   3. Manual Solid Phase Synthesis of Glutathione Analogs

      • Ursel Soomets, Mihkel Zilmer, lo Langel

      Pages 241-257

5. Back Matter

   Pages 259-262

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Hands-on experts describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate their numerous applications. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included.

• Alanin
• Amino acid
• Ligation
• nucleic acid
• protein
• proteins
• signal transduction
• synthesis

• Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK

  John Howl

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