Overview
- Editors:
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Frances H. Arnold
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California Institute of Technology, Pasadena
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George Georgiou
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University of Texas at Austin, Austin
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Table of contents (22 protocols)
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Mutagenesis and Recombination Methods
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- Patrick C. Cirino, Kimberly M. Mayer, Daisuke Umeno
Pages 3-9
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- Thomas Bulter, Miguel Alcalde
Pages 17-22
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- Paul H. Bessette, Marco A. Mena, Annalee W. Nguyen, Patrick S. Daugherty
Pages 29-37
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- Annalee W. Nguyen, Patrick S. Daugherty
Pages 39-44
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- Olga Selifonova, Volker Schellenberger
Pages 45-52
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- Hiroshi Murakami, Takahiro Hohsaka, Masahiko Sisido
Pages 53-64
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- Jessica L. Sneeden, Lawrence A. Loeb
Pages 65-73
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- Radu Georgescu, Geethani Bandara, Lianhong Sun
Pages 75-83
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- Wenjuan Zha, Tongbo Zhu, Huimin Zhao
Pages 91-97
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- Olga Esteban, Ryan D. Woodyer, Huimin Zhao
Pages 99-104
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- Anna Marie Aguinaldo, Frances H. Arnold
Pages 105-110
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- Marc Ostermeier, Stefan Lutz
Pages 129-141
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- Stefan Lutz, Marc Ostermeier
Pages 143-151
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- Andrew K. Udit, Jonathan J. Silberg, Volker Sieber
Pages 153-163
About this book
Biological systems are very special substrates for engineering—uniquely the products of evolution, they are easily redesigned by similar approaches. A simple algorithm of iterative cycles of diversification and selection, evolution works at all scales, from single molecules to whole ecosystems. In the little more than a decade since the first reported applications of evolutionary design to enzyme engineering, directed evolution has matured to the point where it now represents the centerpiece of industrial biocatalyst development and is being practiced by thousands of academic and industrial scientists in com- nies and universities around the world. The appeal of directed evolution is easy to understand: it is conceptually straightforward, it can be practiced without any special instrumentation and, most important, it frequently yields useful solutions, many of which are totally unanticipated. Directed evolution has r- dered protein engineering readily accessible to a broad audience of scientists and engineers who wish to tailor a myriad of protein properties, including th- mal and solvent stability, enzyme selectivity, specific activity, protease s- ceptibility, allosteric control of protein function, ligand binding, transcriptional activation, and solubility. Furthermore, the range of applications has expanded to the engineering of more complex functions such as those performed by m- tiple proteins acting in concert (in biosynthetic pathways) or as part of mac- molecular complexes and biological networks.
Editors and Affiliations
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California Institute of Technology, Pasadena
Frances H. Arnold
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University of Texas at Austin, Austin
George Georgiou
