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Cartilage and Osteoarthritis

  • Book
  • © 2004

Overview

Editors:
  1. Massimo Sabatini

    1. Division de Rhumatologie, Institut de Recherches Servier, Suresnes, France

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  2. Philippe Pastoureau

    1. Division de Rhumatologie, Institut de Recherches Servier, Suresnes, France

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    You can also search for this editor in PubMed   Google Scholar

  3. Frédéric Ceuninck

    1. Division de Rhumatologie, Institut de Recherches Servier, Suresnes, France

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 100)

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Table of contents (22 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Culture and Phenotyping of Chondrocytes in Primary Culture

    • Sylvie Thirion, Francis Berenbaum
    Pages 1-14

  3. Culture of Chondrocytes in Alginate Beads

    • Frédéric De Ceuninck, Christophe Lesur, Philippe Pastoureau, Audrey Caliez, Massimo Sabatini
    Pages 15-22

  4. Immortalization of Human Articular Chondrocytes for Generation of Stable, Differentiated Cell Lines

    • Mary B. Goldring
    Pages 23-35

  5. Culture of Immortalized Chondrocytes and Their Use As Models of Chondrocyte Function

    • Mary B. Goldring
    Pages 37-52

  6. Generation of Pluripotent Stem Cells and Their Differentiation to the Chondrocytic Phenotype

    • Luis A. Solchaga, Jean F. Welter, Donald P. Lennon, Arnold I. Caplan
    Pages 53-67

  7. Semiquantitative Analysis of Gene Expression in Cultured Chondrocytes by RT-PCR

    • Gaëlle Rolland-Valognes
    Pages 69-78

  8. Quantification of mRNA Expression Levels in Articular Chondrocytes With PCR Technologies

    • Audrey McAlinden, Jochen Haag, Brigitte Bau, Pia M. Gebhard, Thomas Aigner
    Pages 79-100

  9. RNA Extraction From Cartilage

    • Frédéric Mallein-Gerin, Jérôme Gouttenoire
    Pages 101-104

  10. Gene Expression Analysis in Cartilage by In Situ Hybridization

    • Frédéric Mallein-Gerin, Jérôme Gouttenoire
    Pages 105-108

  11. Analysis of Differential Gene Expression in Healthy and Osteoarthritic Cartilage and Isolated Chondrocytes by Microarray Analysis

    • Thomas Aigner, Joachim Saas, Alexander Zien, Ralf Zimmer, Pia M. Gebhard, Thomas Knorr
    Pages 109-127

  12. High-Efficiency Nonviral Transfection of Primary Chondrocytes

    • Jean F. Welter, Luis A. Solchaga, Matthew C. Stewart
    Pages 129-145

  13. In Vitro Gene Transfer to Chondrocytes and Synovial Fibroblasts by Adenoviral Vectors

    • Jean-Noel Gouze, Martin J. Stoddart, Elvire Gouze, Glyn D. Palmer, Steven C. Ghivizzani, Alan J. Grodzinsky et al.
    Pages 147-163

  14. Changes of Chondrocyte Metabolism In Vitro

    • Anne-Marie Freyria, Michel Becchi
    Pages 165-182

  15. Analysis of Chondrocyte Functional Markers and Pericellular Matrix Components by Flow Cytometry

    • Gust Verbruggen, Jun Wang, Lai Wang, Dirk Elewaut, Eric M. Veys
    Pages 183-208

  16. A Simple and Reliable Assay of Proteoglycan Synthesis by Cultured Chondrocytes

    • Frédéric De Ceuninck, Audrey Caliez
    Pages 209-217

  17. Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants

    • Christophe Lesur, Massimo Sabatini
    Pages 219-236

  18. Production of Antibodies Against Degradative Neoepitopes in Aggrecan

    • John S. Mort, Peter J. Roughley
    Pages 237-249

  19. Immunoassays for Collagens in Chondrocyte and Cartilage Explant Cultures

    • R. Clark Billinghurst, Fackson Mwale, Anthony Hollander, Mirela Ionescu, A. Robin Poole
    Pages 251-273

  20. Detection of Apoptosis in Cartilage In Situ and in Isolated Chondrocytes

    • Darryl D. D’Lima, Klaus Kuhn, Martin K. Lotz
    Pages 275-289
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About this book

Osteoarthritis (OA), the most common form of arthritis, is generally characterized by a slowly progressive degeneration of articular cartilage, particularly in the weight-bearing joints. It has a stronger prevalence in women, and its incidence increases with age. OA is a major and growing health concern in developed countries, owing to steadily increasing life expectancy and the demand for better quality of life. Because of its chronic nature and nonfatal outcome, OA affects the growing population of the elderly over an increasing time span. Moreover, despite its relatively benign character, OA is one of the most disabling diseases; it is responsible for increasing financial and social burdens in terms of medical treatments, forced inactivity, loss of mobility, and dependence. Despite a growing awareness of OA as a medical problem that has yet to reach its maximum impact on society, there is a surprising absence of effective medical treatments beyond pain control and surgery. So far, only symptom-modifying drugs are available, while there remains a major demand for disease-modifying treatments of proven clinical efficacy. This demand will hopefully be met in the future by some of the drugs that have been pressed into development and are now at different stages of clinical investigation. Nevertheless, the current lack of effective treatments reflects a still insufficient knowledge of cartilage with respect to its metabolism, interactions with other joint tissues, and causes and mechanisms (possibly of very different nature) leading to failure of its turnover.

Editors and Affiliations

  • Division de Rhumatologie, Institut de Recherches Servier, Suresnes, France

    Massimo Sabatini, Philippe Pastoureau, Frédéric Ceuninck

Bibliographic Information

  • Book Title: Cartilage and Osteoarthritis

  • Editors: Massimo Sabatini, Philippe Pastoureau, Frédéric Ceuninck

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592598102

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-247-6Published: 28 July 2004

  • Softcover ISBN: 978-1-61737-450-0Published: 10 November 2010

  • eBook ISBN: 978-1-59259-810-6Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XIV, 358

  • Topics: Endocrinology

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