Overview
- Editors:
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Aviv Hassid
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Department of Physiology, College of Medicine, University of Tennessee, Memphis
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Table of contents (18 protocols)
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- Reinhard Berkels, Svenja Purol-Schnabel, Renate Roesen
Pages 1-8
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- Edward Morcos, N. Peter Wiklund
Pages 21-34
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- Yoshiichiro Kitamura, Hirosuke Kobayashi, Kazuo Tanishita, Kotaro Oka
Pages 35-43
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- Peter A. Steerenberg, Jan G. C. van Amsterdam
Pages 45-68
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- Xiaoxiang Zheng, Gangmin Ning, Dihui Hong, Mu Zhang
Pages 69-80
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- Hazel Sutherland, Olga Zolle, Roba Khundkar, Alec W. M. Simpson, Jonathan C. Jarvis, Stanley Salmons
Pages 105-111
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- Paul R. Gardner, Anne M. Gardner, Craig K. Hallstrom
Pages 133-150
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- Kulwant S. Aulak, Thomas Koeck, John W. Crabb, Dennis J. Stuehr
Pages 151-165
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- Andrew J. Gow, Christiana W. Davis, David Munson, Harry Ischiropoulos
Pages 167-172
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- Trinity J. Bivalacqua, Weiwen Deng, Hunter C. Champion, Wayne J. G. Hellstrom, Philip J. Kadowitz
Pages 173-185
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- Yoshitaka Hirooka, Koji Sakai
Pages 187-200
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- Yoshikazu Maeda, Kazuyuki Shimada, Uichi Ikeda
Pages 213-224
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- Kathleen G. Raman, Richard A. Shapiro, Edith Tzeng, Melina R. Kibbe
Pages 225-234
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Back Matter
Pages 235-246
About this book
Carrying on the high standards of the much-praised first edition of Nitric Oxide Protocols, Aviv Hassid has brought together a panel of expert researchers and clinician scientists to describe in step-by-step detail the latest methodologies for the measurement of nitric oxide--and the enzyme that produces it-in biological tissues and fluids. The authors take advantage of the latest methodologies for the quantitation of biological fluids and tissues, including capillary electrophoresis, microcoaxial electrodes, in vivo measurement of nitric oxide in exhaled air, confocal microscopy, gas chromatography, in situ hybridization, and real-time polymerase chain reaction. Chapters on the measurement of the novel products of nitric oxide, such as nitrated proteins, S-nitrosylated proteins, and dioxygen-dependent NO metabolism, are also included. Additional chapters address the expression of nitric oxide synthase via the use of viral vectors in gene therapy for erectile dysfunction and cancer, as well as in retrovirus, adenovirus, or adenoassociated virus-mediated expression of nitric oxide synthase in vivo. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Nitric Oxide Protocols, 2nd ed., offers investigators and clinican/scientists a gold-standard collection of readily reproducible analytical techniques for measuring levels of nitric oxide and determining its manifold functions and effects.
Reviews
From reviews of the first edition...
"...an excellent book...offers newcomers and experienced scientists a wealth of important practical information that will be of inestimable value both to newcomers and experienced research groups...an essential resource for every laboratory involved in NO-related work..." -Angewandte Chemie
"...a very useful handbook and definitely a must for researchers working at the bench in any field related one way or another to NO." -Psychoneuroendocrinology
"...This book is a must for laboratories with an interest in nitric oxide..." -British Journal of Anesthesia
"...will certainly find its way into many laboratories entering the exciting field of nitric oxide research. " -Quarterly Review of Biology
Editors and Affiliations
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Department of Physiology, College of Medicine, University of Tennessee, Memphis
Aviv Hassid