Overview
- Editors:
-
-
Teresa S. Hawley
-
American Red Cross, Rockville
-
Robert G. Hawley
-
American Red Cross, Rockville
The George Washington University, Washington, DC
Access this book
Other ways to access
Table of contents (24 protocols)
-
Front Matter
Pages i-xiii
-
-
- Howard M. Shapiro, Gerhard Nebe-von-Caron
Pages 33-43
-
- Carleton C. Stewart, Sigrid J. Stewart
Pages 45-65
-
- Omar D. Perez, Peter O. Krutzik, Garry P. Nolan
Pages 67-94
-
-
- Alice L. Givan, Jan L. Fisher, Mary G. Waugh, Nadège Bercovici, Paul K. Wallace
Pages 109-124
-
- Luzheng Liu, Beverly Z. Packard, Martin J. Brown, Akira Komoriya, Mark B. Feinberg
Pages 125-139
-
- William G. Telford, Akira Komoriya, Beverly Z. Packard
Pages 141-159
-
- Shannon S. Eaker, Teresa S. Hawley, Ali Ramezani, Robert G. Hawley
Pages 161-180
-
- Ivan Bertoncello, Brenda Williams
Pages 181-200
-
- Douglas C. Dooley, Barbara K. Oppenlander
Pages 201-217
-
- Teresa S. Hawley, Donald J. Herbert, Shannon S. Eaker, Robert G. Hawley
Pages 219-237
-
- Steven C. Pruitt, Lawrence M. Mielnicki, Carleton C. Stewart
Pages 239-258
-
- David W. Galbraith, Rangasamy Elumalai, Fang Cheng Gong
Pages 259-279
-
- Francis Ka-Ming Chan, Kevin L. Holmes
Pages 281-292
-
- Sam Lievens, José Van der Heyden, Els Vertenten, Jean Plum, Joël Vandekerckhove, Jan Tavernier
Pages 293-309
-
- Michael Feldhaus, Robert Siegel
Pages 311-332
-
- Marielle Cavrois, Jason Neidleman, Martin Bigos, Warner C. Greene
Pages 333-343
-
About this book
Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).
Reviews
"This is a valuable addition to the literature and will be of benefit to research scientists and clinicians whether they be experienced or new to the methodology." - Cell Biochemistry and Function
From Reviews of the First Edition...
"...a well and precisely written collection of protocols which provide important information for both experienced and novice flow cytometry users." -FEBS Letters
"...There are many people who would benefit from reading this book, from those currently using cytometry...to molecular biologists interested in looking at DNA or RNA in the context of cell phenotype in mixed cell populations and those involved in diagnostic testing..." -Molecular Biotechnology
