Overview
- Editors:
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Mouldy Sioud
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Institute of Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
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Table of contents (46 protocols)
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- Rajesh K. Gaur, Andreas Hanne, Guido Krupp
Pages 9-17
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- Kumud K. Singh, Andreas Hanne, Guido Krupp
Pages 33-48
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- Dagmar Klein, Camillo Ricordi, Ricardo L. Pastori
Pages 49-56
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- Soumitra Basu, Catherine Pazsint, Gopeswar Chowdhury
Pages 57-75
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- Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, Arthur D. Riggs
Pages 109-123
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- Wei-Hua Pan, Gary A. Clawson
Pages 125-144
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- Adam Roth, Ronald R. Breaker
Pages 145-164
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- Yasuo Komatsu, Eiko Ohtsuka
Pages 165-177
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- Emma T. Bowden, Anna T. Riegel
Pages 179-194
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- Masayuki Sano, Kazunari Taira
Pages 195-207
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- Roger Abounader, Robert Montgomery, Harry Dietz, John Laterra
Pages 209-219
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- Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, William W. Hauswirth
Pages 221-236
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- Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, Kazunari Taira
Pages 237-243
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- Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, Kazunari Taira
Pages 245-256
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- Mayu Iyo, Hiroaki Kawasaki, Kazunari Taira
Pages 257-265
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- Murray J. Cairns, Lun-Quan Sun
Pages 267-277
About this book
In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, hammerhead ribozymes and derivatives, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, RNase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozyme protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequence-specific gene silencing in a wide variety of organisms. Additional techniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each proven protocol includes a background introduction outlining the principle behind the technique, step-by-step instructions, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Ribozymes and siRNA Protocols details for experienced and novice investigators alike the many exciting advances in our understanding of nucleic acid enzymes, as well as demonstrating how they may be used to analyze gene function and target validation, and to productively develop novel therapeutics for human diseases.
Reviews
"...a timely and important addition to the literature in this rapidly expanding field...The vast scope of the book will appeal to experienced researchers as a source for new avenues of study, and as a central reference for the latest technologies."
-Biochemist
"...will be very useful for both novices and experts as a comprehensive collection of ribozyme protocols..." -Doody's Health Sciences Book Review Journal
"...will provide a valuable source of reference for many laboratories..."
-Microbiology Today
Editors and Affiliations
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Institute of Cancer Research, The Norwegian Radium Hospital, Oslo, Norway
Mouldy Sioud
