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Germ Cell Protocols

Volume 1: Sperm and Oocyte Analysis

  • Book
  • © 2004

Overview

Editors:
  1. Heide Schatten

    1. Department of Veterinary Pathobiology, University of Missouri—Columbia, Columbia

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 253)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Cryopreservation of Semen of the Salmonidae with Special Reference to Large-Scale Fertilization

    • Franz Lahnsteiner
    Pages 1-12

  3. Fertilization and Sperm Chemotaxis in Ascidians

    • Manabu Yoshida
    Pages 13-25

  4. Assays for Vertebrate Sperm Chemotaxis

    • Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, Douglas E. Chandler
    Pages 27-48

  5. Stallion Spermatozoa Viability

    • Katrina Merkies, Mary M. Buhr
    Pages 49-58

  6. Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by Immunofluorescence, Confocal, and Immunoelectron Microscopy

    • Peter Sutovsky
    Pages 59-77

  7. Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pellucida Proteins

    • Heather R. Burkin, Ana Paula Alves-Vieira, David J. Miller
    Pages 79-94

  8. Detection of Human Papillomavirus DNA in Sperm Using Polymerase Chain Reaction

    • Olufemi A. Olatunbosun, Allison M. Case, Harry G. Deneer
    Pages 95-104

  9. Spermatogonial Stem Cells

    • Karim Nayernia, Manyu Li, Wolfgang Engel
    Pages 105-120

  10. Molecular and Cellular Approaches for Establishment of an In Vitro Spermatogenesis System

    • Karim Nayernia, Wolfgang Engel
    Pages 121-133

  11. Acrosin Activity Assay for the Evaluation of Mammalian Sperm Acrosome Reaction

    • Yehudit Lax, Sara Rubinstein, Haim Breitbart
    Pages 135-140

  12. Isolation of Sea Urchin Sperm Plasma Membranes

    • Kathryn J. Mengerink, Victor D. Vacquier
    Pages 141-150

  13. Detection of Centrosome Structure in Fertilized and Artificially Activated Sea Urchin Eggs Using Immunofluorescence Microscopy and Isolation of Centrosomes Followed by Structural Characterization with Field Emission Scanning Electron Microscopy

    • Heide Schatten, Amitabha Chakrabarti
    Pages 151-164

  14. Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryonic Development

    • Mark W. Tengowski
    Pages 165-199

  15. Immunocytochemical Staining of the Metaphase II Spindle in Mammalian Oocytes

    • Steven F. Mullen, John K. Critser
    Pages 201-213

  16. Spindle Imaging in Living Mammalian Oocytes Using the Liquid-Crystal Polarized Light Microscope and Its Practical Use

    • Wei-Hua Wang, Jimmy Gill, Cathy Boutin
    Pages 215-225

  17. In Vitro Maturation and Fertilization of Pig Oocytes

    • Heng-Yu Fan, Qing-Yuan Sun
    Pages 227-233

  18. In Vitro Maturation and In Vitro Fertilization of Human Oocytes

    • Wei-Hua Wang, Jimmy Gill
    Pages 235-253

  19. In Vitro Maturation and Fertilization of Canine Oocytes

    • Karine Reynaud, Marie Saint-Dizier, Sylvie Chastant-Maillard
    Pages 255-272

  20. Detection and Measurement of Membrane-Bound Protein Tyrosine Kinases in the Zebrafish Egg

    • Wenjun Wu, William H. Kinsey
    Pages 273-283
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Keywords

About this book

All sexually reproducing organisms produce primordial germ cells, a small population of cells that differentiate into gametes of either sex and carry to- potency, an ability to develop into an entire new organism. The study of germ cells has undergone enormous advances in recent years and has entered into an explosive phase of new discoveries with the introduction of transgenic te- nologies and nuclear cloning. Basic knowledge and techniques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and strategies that could later be applied to other less readily available mammalian systems. The discovery of centrosomes in ascidians and sea urchin eggs now benefits studies of fertility and infertility in mammals. External in vitro fertilization, now a common technique in assisted fertilization, has only been possible as a result of numerous studies in lower systems in which external fertilization is natural. Egg activation, first explored in sea urchin and ascidian eggs, now benefits techniques designed to increase cl- ing efficiency in farm and domestic animals. Gene manipulations and molecular methods have added to the possibilities of producing live offspring with en- mous biomedical, ecological, and economic implications. The two volumes on germ cells combine techniques in a variety of d- ferent systems; we have selected those systems that have provided landmarks in advancing our knowledge on germ cells.

Editors and Affiliations

  • Department of Veterinary Pathobiology, University of Missouri—Columbia, Columbia

    Heide Schatten

Bibliographic Information

  • Book Title: Germ Cell Protocols

  • Book Subtitle: Volume 1: Sperm and Oocyte Analysis

  • Editors: Heide Schatten

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597440

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-121-9Published: 15 March 2004

  • Softcover ISBN: 978-1-61737-374-9Published: 01 December 2010

  • eBook ISBN: 978-1-59259-744-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 316

  • Topics: Cell Biology

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