Overview
- Editors:
-
-
Sumitra Deb
-
Medical College of Virginia, Virginia Commonwealth University, Richmond
-
Swati Palit Deb
-
Medical College of Virginia, Virginia Commonwealth University, Richmond
Access this book
Other ways to access
Table of contents (18 protocols)
-
-
- Matthew Holmes, Elizabeth Rosenberg, Kristoffer Valerie
Pages 1-16
-
- Xiu Zhu Sun, John Nguyen, Jamil Momand
Pages 17-28
-
- Melissa L. Dumble, Lawrence A. Donehower, Xiongbin Lu
Pages 29-49
-
- Susanne Stein, Yong-Jig Cho, Roger S. Jackson II, Peng Liang
Pages 51-63
-
- Joanna K. Sax, Wafik S. El-Deiry
Pages 65-71
-
- Katherine E. Roth Stagliano, Evie Carchman, Sumitra Deb
Pages 73-91
-
- Mariano J. Scian, Rebecca Frum, Swati Palit Deb, Sumitra Deb
Pages 93-110
-
- Patrick Dumont, Anthony Della Pietra, Maureen E. Murphy
Pages 111-120
-
- David M. Keller, Shelya X. Zeng, Hua Lu
Pages 121-133
-
- Judith Roth, Matthias Dobbelstein
Pages 135-149
-
- Maria Patricia Molina, Christine Cain, Jill Bargonetti
Pages 151-170
-
- Ashley L. Craig, Susan E. Bray, Lee E. Finlan, Neil M. Kernohan, Ted R. Hupp
Pages 171-202
-
- Motohiro Mihara, Ute M. Moll
Pages 203-209
-
- Troy W. Joseph, Ute M. Moll
Pages 211-217
-
- Susan Erster, Neda Slade, Ute M. Moll
Pages 219-230
-
-
- Michael Berger, Ygal Haupt
Pages 245-256
-
- Rebecca Frum, Swati Palit Deb
Pages 257-267
-
Back Matter
Pages 269-280
About this book
Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal the unique structural features and biochemical functions of this protein. Several unique properties of p53 posed a challenge to understa- ing its normal function in the initial phase of its research. The low levels of p53 in normal cells, its stabilization under situations of genotoxic stress, induction of growth arrest, and apoptosis with stabilization of the protein, obstructed the visibility of its normal, unmutated function. The property of p53 that can sense a promoter and transactivate or inhibit is still not well understood. It is still not known whether it is the absence of the protein that causes tumorigenesis, or if its mutants have a dominant role in inducing cancer. p53 Protocols comprises eighteen chapters for the study of the diverse properties of p53 and related proteins. The methods included are invaluable for delineating the function of other proteins that may function as tumor suppr- sors or growth suppressors. The chapters are not presented in any schematic order, for the importance and diversity of the functions of p53 make it imp- sible to organize them suitably. We have made a sincere effort to collect the methods most useful to those investigators working on tumor suppressors or growth suppressors. The purpose of p53 Protocols is not only to provide investigators with methods to analyze similar biochemical functions, but also to familiarize them with the associated problems that arose during the course of investigations.
