Cell Migration in Inflammation and Immunity

1. Front Matter

   Pages i-xii

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2. Chemotaxis and Interaction with Vascular or Lymphatic Endothelium

   • Silvano Sozzani, Annunciata Vecchi, Paola Allavena, Alberto Mantovani

   Pages 1-15

3. Analysis of Integrin-Dependent Rapid Adhesion Under Laminar-Flow Conditions

   • Carlo Laudanna

   Pages 17-25

4. Posttranslational Processing of Chemokines

   • Paul Proost, Frank Mahieu, Evemie Schutyser, Jo Van Damme

   Pages 27-44

5. Chemotactic Profiling of Lymphocyte Subpopulations

   • Lucia Colantonio, Andrea Iellem, Daniele D’Ambrosio

   Pages 45-52

6. Measurement of the Levels of Polymerized Actin (F-Actin) in Chemokine-Stimulated Lymphocytes and GFP-Coupled cDNA Transfected Lymphoid Cells by Flow Cytometry

   • Miguel Vicente-Manzanares, Mariano VitÓn, Francisco Sánchez-Madrid

   Pages 53-68

7. Evaluation of Rho Family Small G-Protein Activity Induced by Integrin Ligation on Human Leukocytes

   • Angela Gismondi, Fabrizio Mainiero, Angela Santoni

   Pages 69-75

8. Reconstructing Leukocyte Migration in 3D Extracellular Matrix by Time-Lapse Videomicroscopy and Computer-Assisted Tracking

   • Peter Friedl, Eva-B. Bröcker

   Pages 77-90

9. Analyzing Chemotaxis Using Dictyostelium discoideum as a Model System

   • Mark A. Landree, Peter N. Devreotes

   Pages 91-104

10. Conditional Transgenic Models to Study Chemokine Biology

    • Sergio A. Lira, Borna Mehrad, Shu-Cheng Chen, Petronio Zalamea, David J. Kinsley, Maria T. Wiekowski et al.

    Pages 105-122

11. Intravital Microscopy as a Tool for Studying Recruitment and Chemotaxis

    • Denise C. Cara, Paul Kubes

    Pages 123-131

12. Tracking Antigen-Specific Lymphocytes In Vivo

    • Claire L. Adams, Catherine M. Rush, Karen M. Smith, Paul Garside

    Pages 133-146

13. Analysis of Homing-Receptor Expression on Infiltrating Leukocytes in Disease States

    • Margherita Mariani, Paola Panina-Bordignon

    Pages 147-165

14. Interaction of Viral Chemokine Inhibitors with Chemokines

    • Antonio Alcami

    Pages 167-180

15. Discovery of Small-Molecule Antagonists of Chemokine Receptors

    • Maria Elena Fuentes

    Pages 181-188

16. Visualization and Analysis of Adhesive Events in Brain Microvessels by Using Intravital Microscopy

    • Gabriela Constantin

    Pages 189-198

17. Animal Models to Study Chemokine Receptor Function In Vivo

    • Clare M. Lloyd, Jose-Carlos Gutierrez-Ramos

    Pages 199-209

18. Assessing the Role of Multiple Phosphoinositide 3-Kinases in Chemokine Signaling

    • Adam P. Curnock, Yannis Sotsios, Stephen G. Ward

    Pages 211-221

19. In Vitro and In Vivo Models to Study Chemokine Regulation of Angiogenesis

    • Giovanni Bernardini, Domenico Ribatti, Gaia Spinetti, Lucia Morbidelli, Marina Ziche, Angela Santoni et al.

    Pages 223-232

20. Real-Time In Vitro Assay for Studying Chemoattractant-Triggered Leukocyte Transendothelial Migration Under Physiological Flow Conditions

    • Guy Cinamon, Ronen Alon

    Pages 233-242

Chemokines and their receptors play a central role in the pathogenesis of numerous, perhaps all, acute and chronic inflammatory diseases. About 50 distinct chemokines produced by a variety cell types and tissues either c- stitutively or in response to inflammatory stimuli are involved in a plethora of biological processes. These small secreted proteins exert their exquisitely variegated functions upon binding to a family of seven-transmembrane spanning G-protein coupled receptors (GPCRs) composed of almost 20 distinct entities. The biological activities of chemokines range from the control of leukocyte trafficking in basal and inflammatory conditions to the regulation of hema- poiesis, angiogenesis, tissue architecture, and organogenesis. The basis for such diversified activities rests, on one hand, upon the ubiquitous nature of chemokine production and chemokine receptor expression. Virtually every cell type can produce chemokines and expresses a unique combination of chemokine receptors. On the other hand, chemokine receptors make use of a flexible and complex network of intracellular signaling machineries that can regulate a variety of cellular functions ranging from cell migration, growth, and differentiation to death. As knowledge of the size of chemokine and chemokine receptor families rapidly reaches completeness, much is still to be uncovered in terms of fu- tional architecture of the chemokine system. The disparity between the large number of chemokines and that smaller number of receptors is balanced by the promiscuity in ligand–receptor interactions, with multiple chemokines binding to the same receptor and several chemokines binding to more than one receptor.

• BioXell S.p.A., Milan, Italy

  Daniele D’Ambrosio, Francesco Sinigaglia

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