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  • Book
  • © 2003

Lentivirus Gene Engineering Protocols

Editors:

  1. Maurizio Federico

    1. Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

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Part of the book series: Methods in Molecular Biology (MIMB, volume 229)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Lentivirus Vectors

    1. Front Matter

      Pages 1-1
      PDF

    2. From Lentiviruses to Lentivirus Vectors

      • Maurizio Federico
      Pages 3-15

    3. The Choice of a Suitable Lentivirus Vector

      • Francesco Lotti, Fulvio Mavilio
      Pages 17-27

    4. Choice and Use of Appropriate Packaging Cell Types

      • Annmarie L. Pacchia, Sayandip Mukherjee, Joseph P. Dougherty
      Pages 29-42

    5. Small- to Large-Scale Production of Lentivirus Vectors

      • Michael P. Marino, Milson J. Luce, Jakob Reiser
      Pages 43-55

    6. Detection and Titration of Lentivirus Vector Preparations

      • Joel Gatlin, Miguel Islas-Ohlmayer, J. Victor Garcia
      Pages 57-68

    8. Detection of Replication-Competent Lentiviral Particles

      • Harry Segall, Richard E. Sutton
      Pages 87-94

  3. Transduction of EX Vivo Cell Cultures Through Lentivirus Vectors

    1. Front Matter

      Pages 95-95
      PDF

    2. Lymphocytes

      • Dhanalakashmi Chinnasamy, Fabio Candotti
      Pages 97-105

    3. Monocyte/Macrophages and Dendritic Cells

      • Harry Segall, Richard E. Sutton
      Pages 107-116

    4. Hematopoietic Stem and Progenitor Cells

      • Robert E. Donahue, Irvin S. Y. Chen
      Pages 117-130

    5. Mesenchymal Stem Cells

      • Xian-Yang Zhang, Vincent F. La Russa, Jakob Reiser
      Pages 131-140

    6. Hippocampal Neurons

      • Jay L. Nadeau
      Pages 141-154

    7. Dorsal Root Ganglia Sensory Neurons

      • Jane Fleming, Samantha L. Ginn, Ian E. Alexander
      Pages 155-167

    8. Cardiomyocytes

      • Dèsirèe Bonci, Michael V. G. Latronico, Gianluigi Condorelli
      Pages 169-179

    9. Airway Epithelia

      • Larry G. Johnson, Scott H. Randell, John C. Olsen
      Pages 181-196

    10. Corneal Cells

      • Binoy Appukuttan, Trevor McFarland, J. Timothy Stout
      Pages 197-203

    11. Retinal Tissue

      • Trevor McFarland, Binoy Appukuttan, J. Timothy Stout
      Pages 205-216
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About this book

Cell gene engineering is emerging as a field with outstanding impact, not only in medicine/biology, but also, and perhaps most importantly, in agriculture and in all those food sciences involved in the fight against world hunger. Lentivirus vector-based technologies represent the last frontier in the development of powerful and reliable methods for both in vitro and in vivo gene transfer in eukaryotic animal cells. Although the design of lentivirus vectors is closely reminiscent of those already successfully applied to the construction of oncoretroviral vectors, some unique features, e.g., the ef- ciency in transducing both postmitotic and stem cells, render the use of lentivirus vectors invaluable. It has been a great pleasure to edit Lentivirus Gene Engineering Pro- cols, owing in part to the high level of enthusiasm that the authors dem- strated in contributing to this book. The fact that so many outstanding scientists engaged in lentivirus vector research have provided articles renders it so- thing more than a technical handbook. In addition to detailed descriptions of the most innovative methodologies, the reader may find very informative ov- views concerning both theoretical and practical aspects of the origin and the development of diverse lentivirus vector types. This, in my opinion, rep- sents a unique added value of this volume, which should help our work resist the passage of time, to which books such as this are particularly sensitive.
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Reviews

"The materials and methods are written in sufficient detail to allow even inexperienced workers to carry out experiments. However, the book would benefit from the inclusion of a section stating the sources of cloning and packaging plasmids. I would recommend this book to all who are planning to work with lentiviral vectors"-SGM Quarterly
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Editors and Affiliations

  • Laboratory of Virology, Istituto Superiore di Sanitá, Rome, Italy

    Maurizio Federico

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Bibliographic Information

  • Book Title: Lentivirus Gene Engineering Protocols

  • Editors: Maurizio Federico

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593933

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • eBook ISBN: 978-1-59259-393-4Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 316

  • Topics: Cell Biology

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eBook USD 74.99
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  • Available as EPUB and PDF
  • Read on any device
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  • Own it forever

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