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  • © 2004

Gene Delivery to Mammalian Cells

Volume 1: Nonviral Gene Transfer Techniques

Editors:

  1. William C. Heiser

    1. Bio-Rad Laboratories, Hercules

    View editor publications

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Part of the book series: Methods in Molecular Biology (MIMB, volume 245)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xv
    PDF

  2. Delivery Using Chemical Methods

    1. Front Matter

      Pages 1-1
      PDF

    2. Chemical Methods for DNA Delivery

      • Dexi Liu, Evelyn F. Chia, Hui Tian
      Pages 3-23

    4. DNA Delivery to Cells in Culture Using Peptides

      • Lei Zhang, Nicholas Ambulos, A.James Mixson
      Pages 33-52

    5. DNA Delivery to Cells in Culture Using PNA Clamps

      • Todd D. Giorgio, Shelby K. Wyatt
      Pages 53-65

    6. Dendrimer-Mediated Cell Transfection in Vitro

      • James R. Baker Jr., Anna U. Bielinska, Jolanta F. Kukowska-Latallo
      Pages 67-81

    7. DNA Delivery to Cells in Culture Using Cationic Liposomes

      • Shelby K. Wyatt, Todd D. Giorgio
      Pages 83-93

    8. Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium

      • John Marshall, Nelson S. Yew, Seng H. Cheng
      Pages 95-114

    9. Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium

      • Young K. Song, Guisheng Zhang, Dexi Liu
      Pages 115-124

    10. Delivery of DNA to Tumor Cells Using Cationic Liposomes

      • Duen-Hwa Yan, Bill Spohn, Mien-Chie Hung
      Pages 125-135

  3. Delivery Using Physical Methods

    1. Front Matter

      Pages 145-145
      PDF

    2. Gene Delivery Using Physical Methods

      • Te-hui W. Chou, Subhabrata Biswas, Shan Lu
      Pages 147-165

    5. Delivery of DNA to Skin by Particle Bombardment

      • Shixia Wang, Swati Joshi, Shan Lu
      Pages 185-196

    8. Delivery of DNA to Skin by Electroporation

      • Nathalie Dujardin, Véronique Préat
      Pages 215-226
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About this book

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo.
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.
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Keywords

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Reviews

For Volumes 1 & 2

"This unrivaled collection of proven, reliable methods for the chemical, physical, and viral delivery of genes into mammalian cells, presented in great detail by experienced researchers, should be a valuable reference work for novices in this filed and experienced researchers alike." - Medical Oncology

Volume 1

"...provides insights into the choices available in this important area of biology and medicine." - Microbiology Today
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Editors and Affiliations

  • Bio-Rad Laboratories, Hercules

    William C. Heiser

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Bibliographic Information

  • Book Title: Gene Delivery to Mammalian Cells

  • Book Subtitle: Volume 1: Nonviral Gene Transfer Techniques

  • Editors: William C. Heiser

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596495

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-086-1Published: 28 October 2003

  • Softcover ISBN: 978-1-61737-349-7Published: 10 November 2010

  • eBook ISBN: 978-1-59259-649-2Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 301

  • Topics: Cell Biology

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Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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