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Cancer Cell Culture

Methods and Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Simon P. Langdon

    1. Western General Hospital Edinburgh, UK

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 88)

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Table of contents (32 protocols)

  1. Front Matter

    Pages i-xii
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  2. Introduction to Cancer Cell Culture

    1. Front Matter

      Pages 1-1
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    2. Basic Principles of Cancer Cell Culture

      • Simon P. Langdon
      Pages 3-15

    3. Essential Techniques of Cancer Cell Culture

      • Kenneth G. Macleod, Simon P. Langdon
      Pages 17-29

  3. Characterization and Authentication

    1. Front Matter

      Pages 31-31
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    2. Characterization and Authentication of Cancer Cell Lines

      • Simon P. Langdon
      Pages 33-42

    3. Authentication of Cancer Cell Lines by DNA Fingerprinting

      • Wilhelm G. Dirks, Hans G. Drexler
      Pages 43-55

    4. Cytogenetic Characterization of Tumor Cell Lines

      • Roderick A. F. MacLeod, Hans G. Drexler
      Pages 57-76

  4. Isolation and Culture of Specialized Cell Types

    1. Front Matter

      Pages 77-77
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    2. Isolation and Culture of Colon Cancer Cell Lines

      • Jae-Gahb Park, Ja-Lok Ku, So-Yeon Park
      Pages 79-92

    3. Isolation and Culture of Melanoma Cell Lines

      • Adam I. Riker
      Pages 93-99

    4. Isolation and Culture of Human Brain Tumor Cells

      • German G. Gomez, Carol A. Kruse
      Pages 101-109

    5. Isolation and Culture of Renal Cancer Cell Lines

      • Jae-Gahb Park, Ja-Lok Ku, So-Yeon Park
      Pages 111-119

    6. Isolation and Culture of Prostate Cancer Cell Lines

      • Nora M. Navone, Matilde Olive, Patricia Troncoso
      Pages 121-132

    7. Isolation and Culture of Ovarian Cancer Cell Lines

      • Simon P. Langdon
      Pages 133-139

    8. Isolation and Culture of Leukemia Cell Lines

      • Hans G. Drexler
      Pages 141-155

  5. Functional Assays in Cell Culture

    1. Front Matter

      Pages 157-157
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    2. Cell Sensitivity Assays: Clonogenic Assay

      • Jane A. Plumb
      Pages 159-164

    3. Cell Sensitivity Assays: The MTT Assay

      • Jane A. Plumb
      Pages 165-169

    4. PARP Cleavage as a Means of Assessing Apoptosis

      • Peter Mullen
      Pages 171-181
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About this book

The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.

Reviews

...I recommend this book as a good starting point to anyone new to cancer cell culture. There are many general principles to be gleaned from the authors' experience...if one gets frustrated after repeated unsuccessful attempts to establish, characterize, and manipulate one's own primary cancer cell line, Dr. Langdon has made sure there is an appendix that catalogues well-established cell lines, and provides the contacts necessary to obtain these cell lines. -- Cancer Biology & Therapy

"...concise and clearly presented...interesting and straightforward information..." - Zellbiolgie Aktuell

"Comprehensive and highly practical...provides both basic scientists and clinical researchers with a gold-standard collection of advanced techniques for culturing cancer cells effectively in their laboratories." - Tumori

Editors and Affiliations

  • Western General Hospital Edinburgh, UK

    Simon P. Langdon

Bibliographic Information

  • Book Title: Cancer Cell Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Simon P. Langdon

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592594069

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • eBook ISBN: 978-1-59259-406-1Published: 01 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: XII, 360

  • Topics: Oncology

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