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Patch-Clamp Analysis

Advanced Techniques

  • Book
  • © 2002

Overview

Editors:
  1. Wolfgang Walz

    1. University of Saskatchewan, Saskatoon, Canada

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    You can also search for this editor in PubMed   Google Scholar

  2. Alan A. Boulton

    1. Alviva Biopharmaceuticals Inc., Saskatoon, Canada

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    You can also search for this editor in PubMed   Google Scholar

  3. Glen B. Baker

    1. University of Alberta, Edmonton, Canada

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    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Neuromethods (NM, volume 35)

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Table of contents (12 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Technology of Patch-Clamp Electrodes

    • Richard A. Levis, James L. Rae
    Pages 1-34

  3. Whole-Cell Patch-Clamp Recordings

    • Harald Sontheimer, Christopher B. Ransom
    Pages 35-67

  4. Single-Channel Recording

    • David J. A. Wyllie
    Pages 69-109

  5. Combined Fluorometric and Electrophysiological Recordings

    • Hartmut Schmidt, Jens Eilers
    Pages 111-134

  6. Patch-Clamp Techniques Applied To Brain Slices

    • James R. Moyer Jr., Thomas H. Brown
    Pages 135-193

  7. Perforated Patch-Clamp Technique

    • Raimondo D’Ambrosio
    Pages 195-216

  8. Fast-Drug Application

    • Manfred Heckmann, Christian Pawlu
    Pages 217-229

  9. Pipet Perfusion Methods

    • Gea-Ny Tseng, Minoru Horie
    Pages 231-244

  10. Patch-Cram Detection of Cyclic GMP in Intact Cells

    • Richard H. Kramer
    Pages 245-264

  11. Loose-Patch Voltage-Clamp Technique

    • Blake D. Anson, William M. Roberts
    Pages 265-286

  12. Recording from Macropatches

    • Frances Moody-Corbett, Mark Fry
    Pages 287-299

  13. Combining Patch-Clamp Techniques with RT-PCR

    • Gerald Seifert, Albert Becker, Christian Steinhäuser
    Pages 301-330

  14. Back Matter

    Pages 331-345
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About this book

Patch-Clamp Analysis: Advanced Techniques describes in reproducible detail all applications that involve patch pipet. Beginning with updated basic patch-clamp techniques, the book presents the newest developments, ranging from fast external solution switching and the switching of the pipet solution during recording, to the loose patch, the perforated patch, and the patch cram detection technique. The advanced techniques covered combine molecular biology and imaging to produce the patch pipet with RT-PCR and fluorometric techniques.

Reviews

From Reviews of an Earlier Version:
"...provides all the information needed to set up and carry out any of the procedures without having to use other resource books...an extremely useful tool..."-FEBS Letters

Editors and Affiliations

  • University of Saskatchewan, Saskatoon, Canada

    Wolfgang Walz

  • Alviva Biopharmaceuticals Inc., Saskatoon, Canada

    Alan A. Boulton

  • University of Alberta, Edmonton, Canada

    Glen B. Baker

Bibliographic Information

  • Book Title: Patch-Clamp Analysis

  • Book Subtitle: Advanced Techniques

  • Editors: Wolfgang Walz, Alan A. Boulton, Glen B. Baker

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1385/1592592767

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media New York 2002

  • eBook ISBN: 978-1-59259-276-0Published: 06 February 2008

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 345

  • Topics: Neurosciences

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