Skip to main content
SpringerLink
Log in
Book cover
  • Book
  • © 2003

Molecular Cytogenetics

Protocols and Applications

Editors:

  1. Yao-Shan Fan

    1. London Health Sciences Center, London
      The University of Western Ontario, Ontario, Canada

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 204)

Sections

Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

This is a preview of subscription content, log in via an institution to check for access.

Table of contents (27 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Basic Concepts and Techniques

    1. Front Matter

      Pages 1-1
      PDF

    2. Molecular Cytogenetics in Medicine

      • Yao-Shan Fan
      Pages 3-20

    3. Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets

      • Larry E. Morrison, Ramesh Ramakrishnan, Teresa M. Ruffalo, Kim A. Wilber
      Pages 21-40

    4. Labeling Fluorescence In Situ Hybridization Probes for RNA Targets

      • Ramesh Ramakrishnan, Larry E. Morrison
      Pages 41-49

    5. Basic FISH Techniques and Troubleshooting

      • Sue Van Stedum, Walter King
      Pages 51-63

  3. Evolving Techniques and Applications

    1. Front Matter

      Pages 65-65
      PDF

    2. Chromosome Microdissection

      • Xin-Yuan Guan
      Pages 67-76

    3. Primed In Situ Labeling

      • Jørn Koch
      Pages 77-84

    4. Spectral Karyotyping

      • Jane Bayani, Jeremy A. Squire
      Pages 85-104

    5. Multicolor FISH

      • Syed M. Jalal, Mark E. Law
      Pages 105-120

    7. Color Banding

      • Vicki L. Hopwood, Christi J. Hada, Armand B. Glassman
      Pages 133-142

    8. Multicolor Fiber FISH

      • Jaennelle Kraan, Anne R. M. von Bergh, Karin Kleiverda, Jan-Willem Vaandrager, Ekaterina S. Jordanova, Anton K. Raap et al.
      Pages 143-153

    9. Multi-Telomere FISH

      • Samantha J. L. Knight, Jonathan Flint
      Pages 155-179

    10. Fluorescence Genotyping for Screening Cryptic Telomeric Rearrangements

      • Laurence Colleaux, Solange Heuertz, Florence Molinari, Marlene Rio
      Pages 181-189

    11. Microarray CGH

      • Ben Beheshti, Paul C. Park, Ilan Braude, Jeremy A. Squire
      Pages 191-207

  4. Special Applications in Chromosomal Disorders

    1. Front Matter

      Pages 209-209
      PDF

    2. Molecular Approaches for Delineating Marker Chromosomes

      • Michael D. Graf, Stuart Schwartz
      Pages 211-218

    3. Interphase FISH for Prenatal Diagnosis of Common Aneuploidies

      • Baruch Feldman, Ayala Aviram-Goldring, Mark I. Evans
      Pages 219-241
Back to top

About this book

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.
Back to top

Reviews

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies...Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories" - Am. J. Hum. Gent.

"Dr. Fan has done a marvelous job of putting together a collection of laboratory protocols and review articles of different FISH technologies. The authors of the different chapters not only gave detailed, easy-to-follow protocols, but also provided their first-hand experiences of troubleshooting. Molecular Cytogenetics: Protocols and Applications is packed with information on cutting-edge FISH technologies and their applications, limitations, and pitfalls, along with detailed laboratory protocols. I would certainly recommend that this book be included in the libraries of cytogeneticists and cytogenetic laboratories." -The American Society of Human Genetics
Back to top

Editors and Affiliations

  • London Health Sciences Center, London

    Yao-Shan Fan

  • The University of Western Ontario, Ontario, Canada

    Yao-Shan Fan

Back to top

Bibliographic Information

  • Book Title: Molecular Cytogenetics

  • Book Subtitle: Protocols and Applications

  • Editors: Yao-Shan Fan

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592593003

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2003

  • Hardcover ISBN: 978-1-58829-006-9Published: 13 September 2002

  • Softcover ISBN: 978-1-61737-300-8Published: 10 November 2010

  • eBook ISBN: 978-1-59259-300-2Published: 05 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 414

  • Topics: Human Genetics

Back to top

Publish with us

Policies and ethics

Back to top
Access via your institution

Buy it now

eBook USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Search

Navigation