Basic Neurobiology Techniques

1. Front Matter

   Pages i-xii

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2. Semiconductor Quantum Dots for Visualization and Sensing in Neuronal Cell Systems

   • Lauren D. Field, Yung Chia Chen, James B. Delehanty

   Pages 1-18

3. Injection of Adeno-Associated Virus Containing Optogenetic and Chemogenetic Probes into the Neonatal Mouse Brain

   • Nhan C. Huynh, Baher A. Ibrahim, Christopher M. Lee, Mickeal N. Key, Daniel A. Llano

   Pages 19-43

4. Guide to Transcranial Imaging of Sound-Evoked Activity in the Auditory Cortex of GCaMP6s Mice In Vivo

   • Georgiy Yudintsev, Christopher M. Lee, Alexander R. Asilador, Daniel A. Llano

   Pages 45-68

5. A Novel Preclinical Rat Model of Alzheimer’s Disease

   • Karim A. Alkadhi

   Pages 69-98

6. Using Zebrafish to Study and Visualize Barriers of the CNS

   • Robyn Anne Umans

   Pages 99-129

7. Photometric Patch Electrode to Simultaneously Measure Neural Electrical Activity and Optical Signal in the Brain Tissue

   • Harunori Ohmori

   Pages 131-153

8. Patch-Clamp Fluorometry and Its Applications to the Study of Ion Channels

   • Esteban Suárez-Delgado, León D. Islas

   Pages 155-183

9. Modeling Early-Onset Postischemic Seizures in Adult and Aging Mice

   • Liang Zhang, Chiping Wu, Justin Wang

   Pages 185-212

10. Modeling Psychological Trauma in Rats

    • Samina Salim

    Pages 213-233

11. Extending the Life Span of Acute Neuronal Tissue for Imaging and Electrophysiological Studies

    • Yossi Buskila, Alba Bellot-Saez, Orsolya Kékesi, Morven Cameron, John Morley

    Pages 235-259

12. Confocal Imaging of Fast Flash Photolysis of Caged Compounds in Cultured Neurons

    • Eduard Korkotian, Menahem Segal

    Pages 261-284

13. Analysis of the Functional States of an Astrocyte Syncytium

    • Yixing Du, Conrad M. Kiyoshi, David Terman, Min Zhou

    Pages 285-313

14. Assessment of Behavioral Dysfunction Following Experimental Traumatic Brain Injury (TBI)

    • Shruti V. Kabadi, Kimberly R. Byrnes

    Pages 315-349

15. Back Matter

    Pages 351-352

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This volume explores the latest techniques and improved methods used to study important neurobiological and associated neuropathological conditions. Chapters cover topics such as the application of semiconductor quantum dots as photostable fluorophores used for labeling and tracking; GCaMP6 transcranial imaging to monitor neural activity; the patch-clamping technique allowing simultaneous monitoring of membrane currents and optical signals; and quantifying behavioral dysfunction caused by traumatic brain injury (TBI). In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and practical, Basic Neurobiology Techniques is a valuable resource for novel and expert researchers interested in expanding their knowledge of this developing field.  

• Xenopus
• Traumatic Brain Injury
• electrophysiology
• optogenetics
• rodents
• neuropathological conditions

• Levine College of Health Sciences, Wingate University, Wingate, USA

  Nicholas J. D. Wright

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