Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2080)
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Table of contents (22 protocols)
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Front Matter
About this book
This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.
Editors and Affiliations
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Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical Centre, Monash University, Clayton, Australia
James Harris, Eric F. Morand
Bibliographic Information
Book Title: Macrophage Migration Inhibitory Factor
Book Subtitle: Methods and Protocols
Editors: James Harris, Eric F. Morand
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9936-1
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9935-4Published: 19 November 2019
Softcover ISBN: 978-1-4939-9938-5Published: 19 November 2020
eBook ISBN: 978-1-4939-9936-1Published: 19 November 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 265
Number of Illustrations: 9 b/w illustrations, 34 illustrations in colour
Topics: Immunology, Cell Biology, Molecular Medicine