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Antibody-Drug Conjugates

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. L. Nathan Tumey

    1. Department of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2078)

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Table of contents (26 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. An Overview of the Current ADC Discovery Landscape

    • L. Nathan Tumey
    Pages 1-22

  3. Pushing the Envelope: Advancement of ADCs Outside of Oncology

    • Michael J. McPherson, Adrian D. Hobson
    Pages 23-36

  4. Conjugations to Endogenous Cysteine Residues

    • Durgesh V. Nadkarni
    Pages 37-49

  5. Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies

    • Pragya Adhikari, Neelie Zacharias, Rachana Ohri, Jack Sadowsky
    Pages 51-69

  6. Transglutaminase-Mediated Conjugations

    • Yasuaki Anami, Kyoji Tsuchikama
    Pages 71-82

  7. Click Chemistry Conjugations

    • Tak Ian Chio, Susan L. Bane
    Pages 83-97

  8. Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual-Labeled Antibody and Fab Conjugates

    • Sujiet Puthenveetil
    Pages 99-112

  9. Bridged Cysteine Conjugations

    • Matthew Bird, Joao Nunes, Mark Frigerio
    Pages 113-129

  10. Antibody Conjugations via Glycosyl Remodeling

    • Hanna Toftevall, Helén Nyhlén, Fredrik Olsson, Jonathan Sjögren
    Pages 131-145

  11. ADC Analysis by Hydrophobic Interaction Chromatography

    • Ryan Fleming
    Pages 147-161

  12. Two-Dimensional Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Analysis of ADCs

    • Soraya Chapel, Florent Rouvière, Morgan Sarrut, Sabine Heinisch
    Pages 163-185

  13. Drug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction

    • Elsa Wagner-Rousset, Olivier Colas, Yannis-Nicolas François, Sabine Heinisch, Davy Guillarme, Sarah Cianférani et al.
    Pages 187-195

  14. Analysis of ADCs by Native Mass Spectrometry

    • Oscar Hernandez-Alba, Anthony Ehkirch, Alain Beck, Sarah Cianférani
    Pages 197-211

  15. High-Resolution Characterization of ADCs by Orbitrap LCMS

    • Jintang He, Surinder Kaur, Keyang Xu
    Pages 213-219

  16. Conjugation Site Analysis by MS/MS Protein Sequencing

    • Linjie Han, Yanqun Zhao, Qunying Zhang
    Pages 221-233

  17. Conjugation Site Analysis of Lysine-Conjugated ADCs

    • Hua Sang, Ning Wan, Gaoyuan Lu, Yang Tian, Guangji Wang, Hui Ye
    Pages 235-250

  18. Characterization of ADCs by Capillary Electrophoresis

    • Wenjing Ning, Yanqun Zhao
    Pages 251-262

  19. Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates Using Capillary Electrophoresis with Mass Spectrometry

    • Josiane Saadé, Rabah Gahoual, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François
    Pages 263-272

  20. Purification of ADCs by Hydrophobic Interaction Chromatography

    • Calvin L. Becker, Robert J. Duffy, Jorge Gandarilla, Steven M. Richter
    Pages 273-290
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Keywords

About this book

 This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the “activation barrier” when undertaking a new discipline, and provides a “toolbox” for the next generation of ADC scientists.


Editors and Affiliations

  • Department of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, USA

    L. Nathan Tumey

Bibliographic Information

  • Book Title: Antibody-Drug Conjugates

  • Book Subtitle: Methods and Protocols

  • Editors: L. Nathan Tumey

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9929-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9928-6Published: 24 October 2019

  • Softcover ISBN: 978-1-4939-9931-6Published: 24 October 2020

  • eBook ISBN: 978-1-4939-9929-3Published: 23 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 373

  • Number of Illustrations: 29 b/w illustrations, 109 illustrations in colour

  • Topics: Antibodies, Pharmacology/Toxicology

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