Antibody-Drug Conjugates

1. Front Matter

   Pages i-xi

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2. An Overview of the Current ADC Discovery Landscape

   • L. Nathan Tumey

   Pages 1-22

3. Pushing the Envelope: Advancement of ADCs Outside of Oncology

   • Michael J. McPherson, Adrian D. Hobson

   Pages 23-36

4. Conjugations to Endogenous Cysteine Residues

   • Durgesh V. Nadkarni

   Pages 37-49

5. Site-Specific Conjugation to Cys-Engineered THIOMAB™ Antibodies

   • Pragya Adhikari, Neelie Zacharias, Rachana Ohri, Jack Sadowsky

   Pages 51-69

6. Transglutaminase-Mediated Conjugations

   • Yasuaki Anami, Kyoji Tsuchikama

   Pages 71-82

7. Click Chemistry Conjugations

   • Tak Ian Chio, Susan L. Bane

   Pages 83-97

8. Utilizing Solid-Phase to Enable High-Throughput, Site-Specific Conjugation and Dual-Labeled Antibody and Fab Conjugates

   • Sujiet Puthenveetil

   Pages 99-112

9. Bridged Cysteine Conjugations

   • Matthew Bird, Joao Nunes, Mark Frigerio

   Pages 113-129

10. Antibody Conjugations via Glycosyl Remodeling

    • Hanna Toftevall, Helén Nyhlén, Fredrik Olsson, Jonathan Sjögren

    Pages 131-145

11. ADC Analysis by Hydrophobic Interaction Chromatography

    • Ryan Fleming

    Pages 147-161

12. Two-Dimensional Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Analysis of ADCs

    • Soraya Chapel, Florent Rouvière, Morgan Sarrut, Sabine Heinisch

    Pages 163-185

13. Drug Loading and Distribution of ADCs After Reduction or IdeS Digestion and Reduction

    • Elsa Wagner-Rousset, Olivier Colas, Yannis-Nicolas François, Sabine Heinisch, Davy Guillarme, Sarah Cianférani et al.

    Pages 187-195

14. Analysis of ADCs by Native Mass Spectrometry

    • Oscar Hernandez-Alba, Anthony Ehkirch, Alain Beck, Sarah Cianférani

    Pages 197-211

15. High-Resolution Characterization of ADCs by Orbitrap LCMS

    • Jintang He, Surinder Kaur, Keyang Xu

    Pages 213-219

16. Conjugation Site Analysis by MS/MS Protein Sequencing

    • Linjie Han, Yanqun Zhao, Qunying Zhang

    Pages 221-233

17. Conjugation Site Analysis of Lysine-Conjugated ADCs

    • Hua Sang, Ning Wan, Gaoyuan Lu, Yang Tian, Guangji Wang, Hui Ye

    Pages 235-250

18. Characterization of ADCs by Capillary Electrophoresis

    • Wenjing Ning, Yanqun Zhao

    Pages 251-262

19. Characterization of the Primary Structure of Cysteine-Linked Antibody-Drug Conjugates Using Capillary Electrophoresis with Mass Spectrometry

    • Josiane Saadé, Rabah Gahoual, Alain Beck, Emmanuelle Leize-Wagner, Yannis-Nicolas François

    Pages 263-272

20. Purification of ADCs by Hydrophobic Interaction Chromatography

    • Calvin L. Becker, Robert J. Duffy, Jorge Gandarilla, Steven M. Richter

    Pages 273-290

 This volume looks at key methodologies that are commonly used across antibody drug conjugates (ADCs) programs. The chapters in this book cover topics such as conjugations to endogenous cysteine residues; click chemistry conjugations; antibody conjugations via glycosyl remodeling; analysis of ADCs by native mass spectrometry; characterization of ADCs by capillary electrophoresis; LC/MS methods for studying lysosomal ADC catabolism; and determination of ADC concentration by ligand-binding assays. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and practical, Antibody-Drug Conjugates: Methods and Protocols is a valuable resource that aims to lower the “activation barrier” when undertaking a new discipline, and provides a “toolbox” for the next generation of ADC scientists.

• ADCs
• Conjugation procedures
• Bioanalysis
• Purification
• Production
• antibodies

• Department of Pharmacy and Pharmaceutical Sciences, Binghamton University, Binghamton, USA

  L. Nathan Tumey

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