Protein Nanotechnology

1. Front Matter

   Pages i-xiii

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2. Introduction to Protein Nanotechnology

   • Laura J. Domigan, Juliet A. Gerrard

   Pages 1-13

3. Design and Production of Protein Building Blocks for Nanotechnology

   1. Front Matter

      Pages 15-15

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   2. Protein Expression in the Baculovirus-Insect Cell Expression System

      • Adetayo A. Adeniyi, Linda Hwee-Lin Lua

      Pages 17-37

   3. Peroxiredoxin Proteins as Building Blocks for Nanotechnology

      • Frankie Conroy, N. Amy Yewdall

      Pages 39-54

   4. Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins

      • Sarah R. Ball, Chi L. L. Pham, Victor Lo, Vanessa K. Morris, Ann H. Kwan, Margaret Sunde

      Pages 55-72

   5. ATP Synthase: Expression, Purification, and Function

      • Meghna Sobti, Robert Ishmukhametov, Alastair G. Stewart

      Pages 73-84

   6. Molecular Superglues: Discovery and Engineering Orthogonalization

      • Paul G. Young, Christopher J. Squire

      Pages 85-99

   7. Production of Multicomponent Protein Templates for the Positioning and Stabilization of Enzymes

      • Samuel Lim, Douglas S. Clark, Dominic J. Glover

      Pages 101-115

4. Towards Protein Nano-Devices

   1. Front Matter

      Pages 117-117

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   2. Adding Function to Protein Scaffolds

      • Kyle Webster, Luigi Sasso, Laura J. Domigan

      Pages 119-147

   3. Virus-Derived Nanoparticles

      • Noor H. Dashti, Frank Sainsbury

      Pages 149-162

   4. Strategies for Increasing Protein Stability

      • Peter G. Chandler, Sebastian S. Broendum, Blake T. Riley, Matthew A. Spence, Colin J. Jackson, Sheena McGowan et al.

      Pages 163-181

   5. Generation of High-Affinity Molecularly Imprinted Nanoparticles for Protein Recognition via a Solid-Phase Synthesis Protocol

      • Francesco Canfarotta, Sergey A. Piletsky, Nicholas W. Turner

      Pages 183-194

   6. Nanotechnology with S-layer Proteins

      • Bernhard Schuster, Uwe B. Sleytr

      Pages 195-218

5. Meaningful Measurements of Protein Nanocomponents

   1. Front Matter

      Pages 219-219

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   2. Preparation of Proteins and Macromolecular Assemblies for Cryo-electron Microscopy

      • Lou Brillault, Michael J. Landsberg

      Pages 221-246

   3. Atomic Force Microscopy of Proteins

      • Yiran An, Sesha Sarathchandra Manuguri, Jenny Malmström

      Pages 247-285

   4. Native Protein Mass Spectrometry

      • Timothy M. Allison, Mark T. Agasid

      Pages 287-299

   5. Nanoparticle Tracking Analysis of β-Casein Nanocarriers

      • Hikmat Masri, Jared K. Raynes

      Pages 301-309

This third edition volume expands on the previous editions with updated approaches and techniques used to study protein nanotechnology and the future of nanomaterial compositions. This book is organized into Three Parts: Part One looks at recombinant protein expression in insect cells, and methods to produce molecular motors, molecular superglues, and protein templates; Part Two explores functionalization strategies and ways to incorporate functional protein components into nanodevices; Part Three discusses various instrumental techniques used to study protein nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Protein Nanotechnology: Protocols, Instrumentation,and Applications, Third Edition is a valuable resource for any researchers looking to expand their knowledge in this evolving field.
 

• SP1 proteins
• Peroxiredoxin
• S-layer proteins
• Molecular machines
• Protein “superglue

• School of Biological Sciences, The MacDiarmid Institute for Advanced Materials and Nanotechnology, School of Chemical Sciences, University of Auckland, Auckland, New Zealand

  Juliet A. Gerrard

• School of Biological Sciences, Department of Chemical and Materials Engineering, The MacDiarmid Institute for Advanced Materials and Nanotechnology, University of Auckland, Auckland, New Zealand

  Laura J. Domigan

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