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Cereal Genomics

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2072)

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
  2. A Low-Cost High-Throughput Method for Plant Genomic DNA Isolation

    • Prateek Gupta, Hymavathi Salava, Yellamaraju Sreelakshmi, Rameshwar Sharma
    Pages 1-7
  3. DNA Methylation and Transcriptomic Next-Generation Technologies in Cereal Genomics

    • Cynthia G. Soto-Cardinault, Fátima Duarte-Aké, Clelia De-la-Peña, Elsa Góngora-Castillo
    Pages 65-84
  4. Genome-Wide Profiling of Histone Modifications with ChIP-Seq

    • William A. Ricci, Laura Levin, Xiaoyu Zhang
    Pages 101-117
  5. Cereal Circular RNAs (circRNAs): An Overview of the Computational Resources for Identification and Analysis

    • Luis M. Vaschetto, Celso Gaspar Litholdo Jr, Lorena Noelia Sendín, Claudia Mabel Terenti Romero, María Paula Filippone
    Pages 157-163
  6. Emerging Genome Engineering Tools in Crop Research and Breeding

    • Andriy Bilichak, Daniel Gaudet, John Laurie
    Pages 165-181
  7. CRISPR/Cas9-Mediated Targeted Mutagenesis in Wheat Doubled Haploids

    • Alison M. R. Ferrie, Pankaj Bhowmik, Nandhakishore Rajagopalan, Sateesh Kagale
    Pages 183-198
  8. Genetic Transformation of Protoplasts Isolated from Leaves of Lolium temulentum and Lolium perenne

    • Huw R. Davis, Anne L. Maddison, Dylan W. Phillips, Huw D. Jones
    Pages 199-205
  9. Agrobacterium Transformation in the Rice Genome

    • Asuka Nishimura
    Pages 207-216
  10. In Vivo Phosphorylation: Development of Specific Antibodies to Detect the Phosphorylated PEPC Isoform for the C4 Photosynthesis in Zea mays

    • Yoshihisa Ueno, Kumiko Yoshizawa-Kumagaye, Junji Emura, Tomoko Urabe, Taku Yoshiya, Tsuyoshi Furumoto et al.
    Pages 217-240
  11. Back Matter

    Pages 241-247

About this book

The objective of this volume is to detail current technologies associated with cereal genomics, providing a valuable resource for researchers working in breeding and molecular crop improvement programs. Chapters guide readers through high-throughput DNA extraction protocols, crop genetic resources, meta-Quantitative Trait Loci (QTL) analysis, association mapping, next-sequencing generation, transposable element-associated variation, transcriptomics analysis, epigenetic variation, identification of imprinted genes, noncoding RNAs (circular RNAs), genome editing technologies, and post-translational protein phosphorylation.Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and cutting-edge, Cereal Genomics: Methods and Protocols to ensure successful results in the further study of this vital field.


Editors and Affiliations

  • Instituto de Diversidad y Ecología Animal, Consejo Nacional de Investigaciones Científicas y Técnicas (IDEA, CONICET), Universidad Nacional de Córdoba, Córdoba, Argentina

    Luis M. Vaschetto

Bibliographic Information

  • Book Title: Cereal Genomics

  • Book Subtitle: Methods and Protocols

  • Editors: Luis M. Vaschetto

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9865-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9864-7Published: 20 September 2019

  • Softcover ISBN: 978-1-4939-9867-8Published: 20 September 2020

  • eBook ISBN: 978-1-4939-9865-4Published: 20 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 247

  • Number of Illustrations: 9 b/w illustrations, 21 illustrations in colour

  • Topics: Plant Sciences, Plant Genetics and Genomics

Buy it now

Buying options

eBook USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access