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Toxoplasma gondii

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Christopher J. Tonkin

    1. Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2071)

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Table of contents (23 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. What a Difference 30 Years Makes! A Perspective on Changes in Research Methodologies Used to Study Toxoplasma gondii

    • John C. Boothroyd
    Pages 1-25

  3. ToxoDB: Functional Genomics Resource for Toxoplasma and Related Organisms

    • Omar S. Harb, David S. Roos
    Pages 27-47

  4. Isolation and Genotyping of Toxoplasma gondii Strains

    • Chunlei Su, Jitender P. Dubey
    Pages 49-80

  5. Generation of Toxoplasma gondii and Hammondia hammondi Oocysts and Purification of Their Sporozoites for Downstream Manipulation

    • Sarah L. Sokol, Zhee Sheen Wong, Jon P. Boyle, Jitender P. Dubey
    Pages 81-98

  6. Assays for Monitoring Toxoplasma gondii Infectivity in the Laboratory Mouse

    • Qiuling Wang, L. David Sibley
    Pages 99-116

  7. PCR Screening of Toxoplasma gondii Single Clones Directly from 96-Well Plates Without DNA Purification

    • Federica Piro, Vern B. Carruthers, Manlio Di Cristina
    Pages 117-123

  8. CRISPR/Cas9-Mediated Generation of Tetracycline Repressor-Based Inducible Knockdown in Toxoplasma gondii

    • Damien Jacot, Dominique Soldati-Favre
    Pages 125-141

  9. Assessing Rhoptry Secretion in T. gondii

    • Catherine Suarez, Melissa B. Lodoen, Maryse Lebrun
    Pages 143-155

  10. High-Throughput Measurement of Microneme Secretion in Toxoplasma gondii

    • Kevin M. Brown, L. David Sibley, Sebastian Lourido
    Pages 157-169

  11. Plate-Based Quantification of Stimulated Toxoplasma Egress

    • Emily Shortt, Sebastian Lourido
    Pages 171-186

  12. Genetic Indicators for Calcium Signaling Studies in Toxoplasma gondii

    • Stephen A. Vella, Abigail Calixto, Beejan Asady, Zhu-Hong Li, Silvia N. J. Moreno
    Pages 187-207

  13. Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging

    • Georgios Pavlou, Isabelle Tardieux
    Pages 209-220

  14. Experimental Approaches for Examining Apicoplast Biology

    • Marco Biddau, Jana Ovciarikova, Lilach Sheiner
    Pages 221-243

  15. Measuring Solute Transport in Toxoplasma gondii Parasites

    • Esther Rajendran, Kiaran Kirk, Giel G. van Dooren
    Pages 245-268

  16. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo

    • Joshua Mayoral, Manlio Di Cristina, Vern B. Carruthers, Louis M. Weiss
    Pages 269-282

  17. Three-Dimensional Reconstruction of Toxoplasma–Neuron Interactions In Situ

    • Carla M. Cabral, Emily F. Merritt, Anita A. Koshy
    Pages 283-295

  18. Model Systems for Studying Mechanisms of Ocular Toxoplasmosis

    • Justine R. Smith, Liam M. Ashander, Yuefang Ma, Elise Rochet, João M. Furtado
    Pages 297-321

  19. Using BioID for the Identification of Interacting and Proximal Proteins in Subcellular Compartments in Toxoplasma gondii

    • Peter J. Bradley, Shima Rayatpisheh, James A. Wohlschlegel, Santhosh M. Nadipuram
    Pages 323-346

  20. Assays to Evaluate Toxoplasma–Macrophage Interactions

    • Debanjan Mukhopadhyay, Jeroen P. J. Saeij
    Pages 347-370
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Keywords

About this book

This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.



Editors and Affiliations

  • Division of Infectious Disease and Immune Defense, The Walter and Eliza Hall Institute of Medical Research, Melbourne, Australia

    Christopher J. Tonkin

Bibliographic Information

  • Book Title: Toxoplasma gondii

  • Book Subtitle: Methods and Protocols

  • Editors: Christopher J. Tonkin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9857-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9856-2Published: 23 November 2019

  • Softcover ISBN: 978-1-4939-9859-3Published: 23 November 2020

  • eBook ISBN: 978-1-4939-9857-9Published: 22 November 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 470

  • Number of Illustrations: 14 b/w illustrations, 63 illustrations in colour

  • Topics: Immunology

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