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Quantitative Real-Time PCR

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Roberto Biassoni

    1. Molecular Diagnostics, IRCCS, Istituto Giannina Gaslini, Genova, Italy

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  2. Alessandro Raso

    1. ASL3 Sistema Sanitario Regione Liguria, S.C. Laboratorio d’Analisi, Genova, Italy

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2065)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiii
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  2. A Quarter Century of PCR-Applied Techniques and Their Still-Increasing Fields of Use

    • Alessandro Raso, Roberto Biassoni
    Pages 1-4

  3. Parameters for Successful PCR Primer Design

    • Stephen A. Bustin, Reinhold Mueller, Tania Nolan
    Pages 5-22

  4. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing

    • Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, Michael W. Pfaffl
    Pages 23-38

  5. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A) Tailing and 5′ Adaptor Ligation

    • Valentin Vautrot, Isabelle Behm-Ansmant
    Pages 39-54

  6. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PC R

    • Priscilla Biswas, Eddi Di Marco, Mauro S. Malnati
    Pages 55-64

  7. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)

    • Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, Alice Gomes Fernandes-Monteiro
    Pages 65-77

  8. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)

    • Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, Belén Rodelas
    Pages 79-94

  9. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case of Diplodia sapinea on Pine

    • Nicola Luchi, Alberto Santini, Francesca Salvianti, Pamela Pinzani
    Pages 95-104

  10. A General Protocol for Accurate Gene Expression Analysis in Plants

    • Ellen De Keyser, Laurence Desmet, Magali Losschaert, Jan De Riek
    Pages 105-118

  11. Gene Expression Analysis in Bacteria by RT-qPCR

    • Danilo J. P. G. Rocha, Thiago L. P. Castro, Eric R. G. R. Aguiar, Luis G. C. Pacheco
    Pages 119-137

  12. Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR

    • Francesca Salvianti, Filomena Costanza, Gemma Sonnati, Pamela Pinzani
    Pages 139-151

  13. Molecular Monitoring of Chronic Myeloid Leukemia

    • Katherine Dominy, Katya Mokretar, Alistair G. Reid, Jamshid S. Khorashad
    Pages 153-173

  14. Normalization in Human Glioma Tissue

    • Ana Paula Santin Bertoni, Isabele Cristiana Iser, Rafael Paschoal de Campos, Márcia Rosangela Wink
    Pages 175-190

  15. qPCR Applications for the Determination of the Biological Age

    • Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello et al.
    Pages 191-197

  16. QuantStudio™ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis

    • Chiara Broccanello, Letizia Gerace, Piergiorgio Stevanato
    Pages 199-208

  17. Digital PCR and the QuantStudio™ 3D Digital PCR System

    • Marion Laig, Christie Fekete, Nivedita Majumdar
    Pages 209-231

  18. Back Matter

    Pages 233-235
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Keywords

About this book

This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Revised and authoritative, Quantitative Real-Time PCR: Methods and Protocols, Second Edition is an ideal guide to this expanding and vital field of study.

Editors and Affiliations

  • Molecular Diagnostics, IRCCS, Istituto Giannina Gaslini, Genova, Italy

    Roberto Biassoni

  • ASL3 Sistema Sanitario Regione Liguria, S.C. Laboratorio d’Analisi, Genova, Italy

    Alessandro Raso

Bibliographic Information

  • Book Title: Quantitative Real-Time PCR

  • Book Subtitle: Methods and Protocols

  • Editors: Roberto Biassoni, Alessandro Raso

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9833-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9832-6Published: 03 October 2019

  • Softcover ISBN: 978-1-4939-9835-7Published: 03 October 2020

  • eBook ISBN: 978-1-4939-9833-3Published: 02 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 235

  • Number of Illustrations: 15 b/w illustrations, 55 illustrations in colour

  • Topics: Genetics and Genomics, Human Genetics

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