Omics Approaches to Understanding Muscle Biology

1. Front Matter

   Pages i-vii

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2. The Role of Omics Approaches in Muscle Research

   • Stefano Schiaffino, Carlo Reggiani, Marta Murgia

   Pages 1-6

3. Genomic and Epi-genomic

   1. Front Matter

      Pages 7-7

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   2. Genome-Wide Association Studies in Muscle Physiology and Disease

      • Luca Bello, Elena Pegoraro, Eric P. Hoffman

      Pages 9-30

   3. Chromatin Epigenomics in Muscle Development and Disease

      • Jelena Perovanovic

      Pages 31-44

   4. Guidelines for Bioinformatics and the Statistical Analysis of Omic Data

      • Surajit Bhattacharya, Heather Gordish-Dressman

      Pages 45-75

4. Transcriptomic

   1. Front Matter

      Pages 77-77

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   2. Transcriptomic Approaches for Muscle Biology and Disorders

      • Poching Liu, Surajit Bhattacharya, Yi-Wen Chen

      Pages 79-107

   3. Approaches to Studying the microRNAome in Skeletal Muscle

      • Alyson A. Fiorillo, Christopher R. Heier

      Pages 109-133

5. Proteomic

   1. Front Matter

      Pages 135-135

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   2. Proteomic Profiling of Human Skeletal Muscle in Health and Disease

      • Paul R. Langlais, Lawrence J. Mandarino

      Pages 137-154

   3. Proteome Profiling of Muscle Cells and Muscle Tissue Using Stable Isotope Labeling by Amino Acids

      • Emily Canessa, Mansi V. Goswami, Alison M. Samsel, Michael Ogundele, Shefa M. Tawalbeh, Tchilabalo D. Alayi et al.

      Pages 155-169

   4. Investigating Muscle Protein Turnover on a Protein-by-Protein Basis Using Dynamic Proteome Profiling

      • Jatin G. Burniston

      Pages 171-190

6. Metabolomic

   1. Front Matter

      Pages 191-191

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   2. Skeletal Muscle Metabolomics for Metabolic Phenotyping and Biomarker Discovery

      • Kenneth Allen Dyar, Anna Artati, Alexander Cecil, Jerzy Adamski

      Pages 193-217

This book is a collection of principles and current practices in omics research, applied to skeletal muscle physiology and disorders. The various sections are categorized according to the level of biological organization, namely, genomics (DNA), transcriptomics (RNA), proteomics (protein), and metabolomics (metabolite). With skeletal muscle as the unifying theme, and featuring contributions from leading experts in this traditional field of research, it highlights the importance of skeletal muscle tissue in human development, health and successful ageing. It also discusses other fascinating topics like developmental biology, muscular dystrophies, exercise, insulin resistance and atrophy due to disuse, ageing or other muscle diseases, conveying the vast opportunities for generating new hypotheses as well as testing existing hypotheses by combining high-throughput techniques with proper experiment designs, bioinformatics and statistical analyses.

Presenting the latest researchtechniques, this book is a valuable resource for the physiology community, particularly researchers and grad students who want to explore the new opportunities for omics technologies in basic physiology research.

 

• Skeletal muscle
• Skeletal muscle physiology
• skeletal muscle development
• OMICS technologies
• muscular dystrophy
• high-throughput analytical techniques
• RNA profiling
• muscle atrophy and dysfunction
• Proteomics
• Metabolomics
• Systems Biology

• Research Institute for Sport and Exercise Sciences, Liverpool John Moores University, Liverpool, UK

  Jatin George Burniston

• Children’s National Health System, George Washington University, Washington, DC, USA

  Yi-Wen Chen

Jatin Burniston is a Professor of Muscle Proteomics at the Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, UK. He established the first proteomics facility with a specific focus on exercise physiology and published the first works reporting proteomic analysis of striated muscle responses to exercise training. Jatin strives to continue pioneering the field of exercise proteomics and is a proud champion of the application of non-targeted '-omic' research in exercise physiology. He recently combined his expertise in mass spectrometry and proteomics with new metabolic labelling methods using deuterated/ ‘heavy’ water to establish the unique ‘Dynamic Proteome Profiling’ method. Jatin serves on the Editorial Board of the American Physiological Society (APS) journal, Physiological Genomics, and is a Fellow of the European College of Sports Sciences (ECSS) and a member of the ECSS Scientific Committee. He also leads the MSc Exercise Physiology programmeat Liverpool John Moores and particularly enjoys delivering his module on Molecular Exercise Physiology.

Yi-Wen Chen is an Associate Professor of Genomics and Precision Medicine at the George Washington University and Principal Investigator at the Center for Genetic Medicine Research, Children’s National Health System, DC, USA. Her research uses transcriptomic approaches to study molecular pathways in muscles in response to physiological stimuli and pathological conditions. Yi-Wen uses genome-wide RNA profiling to dissect the disease mechanisms of various muscle disorders and to identify molecular mechanisms of adaptive remodelling. In addition, her group uses next-generation sequencing and long-read sequencing to study genetic, epigenetic and transcriptomic changes in muscles using physiological and disease models. Currently she is part of the Human Cell Atlas consortium, with a focus on single-cell profiles and spatial characterization of gene expression in skeletal muscles at different developmental stages.

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