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  • © 2020

Oncolytic Viruses

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2058)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
  2. Introduction to Oncolytic Virotherapy

    • Christine E. Engeland, John C. Bell
    Pages 1-6
  3. Methods for Modification of Therapeutic Viruses

    • Claudia A. P. Hill, Luca Bau, Robert Carlisle
    Pages 7-29
  4. Development of Entry-Targeted Oncolytic Measles Viruses

    • Michael D. Mühlebach, Roberto Cattaneo
    Pages 51-75
  5. Ex Vivo Virotherapy with Myxoma Virus to Treat Cancer

    • Nancy Y. Villa, Lina S. Franco, Grant McFadden
    Pages 95-110
  6. Immunomodulation in Oncolytic Measles Virotherapy

    • Laura Dietz, Christine E. Engeland
    Pages 111-126
  7. A Functional Assay to Determine the Capacity of Oncolytic Viruses to Induce Immunogenic Tumor Cell Death

    • Tiphaine Delaunay, Carole Achard, Marc Grégoire, Frédéric Tangy, Nicolas Boisgerault, Jean-François Fonteneau
    Pages 127-132
  8. Analysis of Immunological Treatment Effects of Virotherapy in Tumor Tissue

    • Krishna Das, Carles Urbiola, Bart Spiesschaert, Philipp Mueller, Guido Wollmann
    Pages 155-177
  9. Detection of Tumor Antigen-Specific T-Cell Responses After Oncolytic Vaccination

    • Jonathan G. Pol, Byram W. Bridle, Brian D. Lichty
    Pages 191-211
  10. Delivery of Oncolytic Reovirus by Cell Carriers

    • Elizabeth J. Ilett
    Pages 229-236
  11. In Vivo Bioimaging for Monitoring Intratumoral Virus Activity

    • Liesa-Marie Schreiber, Carles Urbiola, Patrik Erlmann, Guido Wollmann
    Pages 237-248
  12. Use of Liquid Patient Ascites Fluids as a Preclinical Model for Oncolytic Virus Activity

    • Eleanor M. Scott, Sally Frost, Hena Khalique, Joshua D. Freedman, Len W. Seymour, Janet Lei-Rossmann
    Pages 261-270
  13. Generating Primary Models of Human Cancer to Aid in the Development of Clinically Relevant Oncolytic Viruses

    • Brian A. Keller, Marie-Ève Wedge, Abera Surendran, Carolina S. Ilkow
    Pages 271-284

About this book

This book aims to provide a guide for virologists, translational researchers, and clinicians in the field of cancer research by providing reference protocols and methodologies from vector development through clinical translation.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Oncolytic Viruses: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Editors and Affiliations

  • Research Group Mechanisms of Oncolytic Immunotherapy, Clinical Cooperation Unit Virotherapy, National Center for Tumor Diseases (NCT), German Cancer Research Center (DKFZ), University Hospital Heidelberg, Heidelberg, Germany

    Christine E. Engeland

Bibliographic Information

  • Book Title: Oncolytic Viruses

  • Editors: Christine E. Engeland

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9794-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9793-0Published: 05 September 2019

  • Softcover ISBN: 978-1-4939-9796-1Published: 05 September 2020

  • eBook ISBN: 978-1-4939-9794-7Published: 04 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 329

  • Number of Illustrations: 16 b/w illustrations, 48 illustrations in colour

  • Topics: Cancer Research, Virology

Buy it now

Buying options

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access