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Nitrogen Metabolism in Plants

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Kapuganti Jagadis Gupta

    1. National Institute of Plant Genome Research, New Delhi, India

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2057)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. An Overview of Important Enzymes Involved in Nitrogen Assimilation of Plants

    • Reddy Kishorekumar, Mallesham Bulle, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 1-13

  3. Methods for Measuring Nitrate Reductase, Nitrite Levels, and Nitric Oxide from Plant Tissues

    • Aakanksha Wany, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 15-26

  4. Measurement of Nitrate Reductase Activity in Tomato (Solanum lycopersicum L.) Leaves Under Different Conditions

    • Mallesham Bulle, Reddy Kishorekumar, Pradeep K. Pathak, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 27-35

  5. Fluorimetric-Based Method to Detect and Quantify Total S-Nitrosothiols (SNOs) in Plant Samples

    • Paulo T. Mioto, Alejandra Matiz, Luciano Freschi, Francisco J. Corpas
    Pages 37-43

  6. Measurement of S-Nitrosoglutathione Reductase Activity in Plants

    • Martina Janků, Tereza Tichá, Lenka Luhová, Marek Petřivalský
    Pages 45-59

  7. Expression Analysis of Important Genes Involved in Nitrogen Metabolism Under Hypoxia

    • Mallesham Bulle, Reddy Kishorekumar, Aakanksha Wany, Kapuganti Jagadis Gupta
    Pages 61-69

  8. Enzymatic Conversions of Glutamate and γ-Aminobutyric Acid as Indicators of Plant Stress Response

    • Alexander T. Eprintsev, Natalia V. Selivanova, Abir U. Igamberdiev
    Pages 71-78

  9. Using Different Forms of Nitrogen to Study Hypersensitive Response Elicited by Avirulent Pseudomonas syringae

    • Namrata Singh, Pooja Singh, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 79-92

  10. Using Foldscope to Monitor Superoxide Production and Cell Death During Pathogen Infection in Arabidopsis Under Different Nitrogen Regimes

    • Reena Arora, Pooja Singh, Aprajita Kumari, Pradeep Kumar Pathak, Kapuganti Jagadis Gupta
    Pages 93-102

  11. Methods for Estimation of Nitrogen Components in Plants and Microorganisms

    • Pratiksha Singh, Rajesh Kumar Singh, Qi-Qi Song, Hai-Bi Li, Li-Tao Yang, Yang-Rui Li
    Pages 103-112

  12. A Precise Method for Analyzing Nitrogen Use in Foxtail Millet

    • Tirthankar Bandyopadhyay, Manoj Prasad
    Pages 113-118

  13. Methods for Isolation and Characterization of Nitrogen-Fixing Legume-Nodulating Bacteria

    • Nisha Tak, Garima Bissa, Hukam S. Gehlot
    Pages 119-143

  14. Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR

    • Engil I. P. Pereira, Marcelo C. M. Teixeira Filho
    Pages 145-153

  15. Extracellular Matrix Proteome: Isolation of ECM Proteins for Proteomics Studies

    • Eman Elagamey, Kanika Narula, Niranjan Chakraborty, Subhra Chakraborty
    Pages 155-172

  16. Back Matter

    Pages 173-178
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Keywords

About this book

This volume explores several different aspects of nitrogen metabolism, ranging from nitrogen uptake to assimilation. The chapters in this book cover topics such as measurement of activities of enzymes involved in nitrogen metabolism (i.e., nitrate reductase); measurement of nitric oxide, nitrite and ways to detect the N content in plants and microbes; characterization of root nodule bacteria (RNB); and techniques to perform proteomics of Extracellular Matrix (ECM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Thorough and cutting-edge, Nitrogen Metabolism in Plants: Methods and Protocols is a valuable tool for novice and expert researchers who are interested in learning more about this evolving field.



Editors and Affiliations

  • National Institute of Plant Genome Research, New Delhi, India

    Kapuganti Jagadis Gupta

Bibliographic Information

  • Book Title: Nitrogen Metabolism in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Kapuganti Jagadis Gupta

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9790-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9789-3Published: 09 October 2019

  • Softcover ISBN: 978-1-4939-9792-3Published: 09 October 2020

  • eBook ISBN: 978-1-4939-9790-9Published: 08 October 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 178

  • Number of Illustrations: 9 b/w illustrations, 32 illustrations in colour

  • Topics: Plant Sciences

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