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Electroporation Protocols

Microorganism, Mammalian System, and Nanodevice

  • Book
  • © 2020

Overview

Editors:
  1. Shulin Li

    1. MD Anderson Cancer Center, The University of Texas, Houston, USA

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  2. Lingqian Chang

    1. School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

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  3. Justin Teissie

    1. Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail methods and reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2050)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Design and Fabrication of Micro-/Nano-Electroporation

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Cell Size-Specific Transfection by Micropillar Array Electroporation

      • Xuan Liu, Yingbo Zu, Shengnian Wang
      Pages 3-12

    3. Soft Electroporation Through 3D Hollow Nanoelectrodes

      • Jun Yin, Yang Li
      Pages 13-19

    4. Micromachining of Polymeric Microfluidic Micro/Nanoelectroporation Device

      • Lei Li
      Pages 21-27

    5. 3D Nanochannel Array for High-Throughput Cell Manipulation and Electroporation

      • Lingqian Chang, Stephen Black, Chandani Chitrakar, Mehdi Nouri
      Pages 29-41

    6. Low-Voltage Flow-Through Electroporation Membrane and Method

      • Juliette Experton, Aaron G. Wilson, Charles R. Martin
      Pages 43-55

  3. Micro-/Nano-Electroporation for In Vitro Delivery

    1. Front Matter

      Pages 57-57
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    2. Nanofountain Probe Electroporation for Monoclonal Cell Line Generation

      • Horacio D. Espinosa, Prithvijit Mukherjee, Cesar Patino
      Pages 59-68

    3. 3D Nanochannel Electroporation for Macromolecular Nucleotide Delivery

      • Lingqian Chang, Chandani Chitrakar, Mehdi Nouri
      Pages 69-77

    4. Nanoelectroporation and Collection of Genetically Modified Exosomes in Primary Cultures of Dendritic Cells

      • Silvia Duarte-Sanmiguel, Natalia Higuita-Castro, Daniel Gallego-Perez
      Pages 79-84

    5. Electroporation of CRISPR-Cas9 into Malignant B Cells for Loss-of-Function Studies of Target Gene Via Knockout

      • Jian Yu
      Pages 85-90

    6. Microfluidic Device for Localized Electroporation

      • Justin Brooks, Arian Jaberi, Ruiguo Yang
      Pages 91-97

  4. Advanced In Vivo Electroporation

    1. Front Matter

      Pages 99-99
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    2. Transdermal Delivery of Nucleic Acid Mediated by Punching and Electroporation

      • Dong Huang, Yuanyu Huang, Zhihong Li
      Pages 101-112

    3. Targeted In Vivo Electroporation Using Nanoengineered Microelectrodes

      • Daniel Schwarz, Andreas T. Schaefer
      Pages 113-120

    4. Zygote Electroporation for CRISPR/Cas9 Delivery to Generate Genetically Modified Mice

      • Tatsuya Takemoto
      Pages 121-126

    5. Genome Editing in Staphylococcus aureus by Conditional Recombineering and CRISPR/Cas9-Mediated Counterselection

      • Kelsi Penewit, Stephen J. Salipante
      Pages 127-143

    6. Isolation and Nanoscale Electroporation of Primary Neuronal Cultures In Situ

      • Diego Alzate-Correa, William Lawrence, Natalia Higuita-Castro, Daniel Gallego-Perez
      Pages 145-152

  5. Microorganism Electroporation

    1. Front Matter

      Pages 153-153
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Keywords

About this book

This third edition provides in-depth knowledge on the delivery of naked DNA and small-interfering RNA (siRNA) to the targeted microorganism, mammalian single cells, tissues, and animals for prevention and treatment of disease. It builds on the success of the first edition and on the progress made in siRNA delivery and DNA vaccines for large animals as well as discovery of electroporation applications for the fragile tissues and for internal organs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and easily accessible, Electroporation Protocols: Microorganism, Mammalian System, and Nanodevice, Third Edition aims to be an invaluable resource for investigators both in and outside of this field. 

Editors and Affiliations

  • MD Anderson Cancer Center, The University of Texas, Houston, USA

    Shulin Li

  • School of Biological Science and Medical Engineering, Beihang University, Beijing, China, Institute of Nanotechnology for Single Cell Analysis (INSCA), Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University,, Beijing, China

    Lingqian Chang

  • Institut de Pharmacologie et de Biologie Structurale, Université de Toulouse, CNRS, UPS, Toulouse, France

    Justin Teissie

Bibliographic Information

  • Book Title: Electroporation Protocols

  • Book Subtitle: Microorganism, Mammalian System, and Nanodevice

  • Editors: Shulin Li, Lingqian Chang, Justin Teissie

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9740-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9739-8Published: 30 August 2019

  • Softcover ISBN: 978-1-4939-9742-8Published: 30 August 2020

  • eBook ISBN: 978-1-4939-9740-4Published: 29 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 3

  • Number of Pages: XI, 196

  • Number of Illustrations: 7 b/w illustrations, 31 illustrations in colour

  • Topics: Gene Therapy

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