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  • Book
  • © 2020

Brain Development

Methods and Protocols

Editors:

  1. Simon G. Sprecher

    1. Department of Biology, University of Fribourg, Fribourg, Switzerland

    View editor publications

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2047)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    PDF

  2. Invertebrate Models

    1. Front Matter

      Pages 1-1
      PDF

    2. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra

      • Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, Brigitte Galliot
      Pages 3-24

    5. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

      • Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez
      Pages 59-80

    6. Immunostaining of the Embryonic and Larval Drosophila Brain

      • Frank Hirth, Danielle C. Diaper
      Pages 81-96

    9. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update

      • Emma L. Powell, Iris Salecker
      Pages 137-152

    10. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

      • Karolina Miszczak, Boris Egger
      Pages 153-160

    11. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila

      • Cornelia Fritsch, Simon G. Sprecher
      Pages 161-189

    12. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development

      • Max S. Farnworth, Kolja N. Eckermann, Hassan M. M. Ahmed, Dominik S. Mühlen, Bicheng He, Gregor Bucher
      Pages 191-217

    14. Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium

      • Vera S. Hunnekuhl, Janna Siemanowski, Max S. Farnworth, Bicheng He, Gregor Bucher
      Pages 233-251

    15. X-Ray Microscopy of the Larval Crustacean Brain

      • Jakob Krieger, Franziska Spitzner
      Pages 253-270

    18. Methods in Brain Development of Molluscs

      • Andreas Wanninger, Tim Wollesen
      Pages 311-324
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About this book

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizingrecent technical advances in molecular genetics for the study of the brain.
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Keywords

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Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G. Sprecher

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Bibliographic Information

  • Book Title: Brain Development

  • Book Subtitle: Methods and Protocols

  • Editors: Simon G. Sprecher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9732-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9731-2Published: 26 September 2019

  • Softcover ISBN: 978-1-4939-9734-3Published: 26 September 2020

  • eBook ISBN: 978-1-4939-9732-9Published: 24 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 527

  • Number of Illustrations: 20 b/w illustrations, 96 illustrations in colour

  • Topics: Neurobiology, Developmental Biology, Neurosciences, Animal Models

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Access via your institution

Buy it now

eBook USD 169.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 219.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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