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Brain Development

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Simon G. Sprecher

    1. Department of Biology, University of Fribourg, Fribourg, Switzerland

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2047)

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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xiv
    Download chapter PDF

  2. Invertebrate Models

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Combining BrdU-Labeling to Detection of Neuronal Markers to Monitor Adult Neurogenesis in Hydra

      • Wanda Buzgariu, Marie-Laure Curchod, Chrystelle Perruchoud, Brigitte Galliot
      Pages 3-24

    3. Reverse Genetic Approaches to Investigate the Neurobiology of the Cnidarian Sea Anemone Nematostella vectensis

      • Jamie A. Havrilak, Michael J. Layden
      Pages 25-43

    4. Generating Transgenic Reporter Lines for Studying Nervous System Development in the Cnidarian Nematostella vectensis

      • Fabian Rentzsch, Eduard Renfer, Ulrich Technau
      Pages 45-57

    5. Immunostaining and In Situ Hybridization of the Developing Acoel Nervous System

      • Elena Perea-Atienza, Brenda Gavilán, Simon G. Sprecher, Pedro Martinez
      Pages 59-80

    6. Immunostaining of the Embryonic and Larval Drosophila Brain

      • Frank Hirth, Danielle C. Diaper
      Pages 81-96

    7. Nonfluorescent RNA In Situ Hybridization Combined with Antibody Staining to Visualize Multiple Gene Expression Patterns in the Embryonic Brain of Drosophila

      • David Jussen, Rolf Urbach
      Pages 97-113

    8. Analysis of Complete Neuroblast Cell Lineages in the Drosophila Embryonic Brain via DiI Labeling

      • Karoline F. Kraft, Rolf Urbach
      Pages 115-135

    9. Flybow to Dissect Circuit Assembly in the Drosophila Brain: An Update

      • Emma L. Powell, Iris Salecker
      Pages 137-152

    10. Live Cell Imaging of Neural Stem Cells in the Drosophila Larval Brain

      • Karolina Miszczak, Boris Egger
      Pages 153-160

    11. CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila

      • Cornelia Fritsch, Simon G. Sprecher
      Pages 161-189

    12. The Red Flour Beetle as Model for Comparative Neural Development: Genome Editing to Mark Neural Cells in Tribolium Brain Development

      • Max S. Farnworth, Kolja N. Eckermann, Hassan M. M. Ahmed, Dominik S. Mühlen, Bicheng He, Gregor Bucher
      Pages 191-217

    13. A Protocol for Double Fluorescent In Situ Hybridization and Immunohistochemistry for the Study of Embryonic Brain Development in Tribolium castaneum

      • Marita Buescher, Georg Oberhofer, Natalia Carolina Garcia-Perez, Gregor Bucher
      Pages 219-232

    14. Immunohistochemistry and Fluorescent Whole Mount RNA In Situ Hybridization in Larval and Adult Brains of Tribolium

      • Vera S. Hunnekuhl, Janna Siemanowski, Max S. Farnworth, Bicheng He, Gregor Bucher
      Pages 233-251

    15. X-Ray Microscopy of the Larval Crustacean Brain

      • Jakob Krieger, Franziska Spitzner
      Pages 253-270

    16. Immunolocalization of Neurotransmitters and Neuromodulators in the Developing Crayfish Brain

      • Steffen Harzsch, Caroline Viertel
      Pages 271-291

    17. Immunostainings in Nervous System Development of the Nematode C. elegans

      • Janet S. Duerr
      Pages 293-310

    18. Methods in Brain Development of Molluscs

      • Andreas Wanninger, Tim Wollesen
      Pages 311-324

    19. A Simple Method to Identify Ascidian Brain Lineage Cells at Neural Plate Stages Following In Situ Hybridization

      • Clare Hudson
      Pages 325-345
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Keywords

About this book

This book provides a thorough introduction to widely used techniques for the study of the intersection between developmental biology and neuroscience, an exceptional area to address and investigate impacting biological questions. The fully updated volume examines cutting-edge techniques on a representative range of animals, including widely used genetic model systems, such as the fruit fly, zebra fish, chicken, and mouse, as well as non-canonical experimental systems opened up through the advent of genome editing.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Brain Development: Methods and Protocols, Second Edition is an ideal guide for researchers interested in utilizingrecent technical advances in molecular genetics for the study of the brain.

Editors and Affiliations

  • Department of Biology, University of Fribourg, Fribourg, Switzerland

    Simon G. Sprecher

Bibliographic Information

  • Book Title: Brain Development

  • Book Subtitle: Methods and Protocols

  • Editors: Simon G. Sprecher

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9732-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9731-2Published: 26 September 2019

  • Softcover ISBN: 978-1-4939-9734-3Published: 26 September 2020

  • eBook ISBN: 978-1-4939-9732-9Published: 24 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIV, 527

  • Number of Illustrations: 20 b/w illustrations, 96 illustrations in colour

  • Topics: Neurobiology, Developmental Biology, Neurosciences, Animal Models

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