Editors:
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2041)
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Table of contents (27 protocols)
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Front Matter
About this book
This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide asound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.
Editors and Affiliations
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BioMedical Research Institute of Murcia (IMIB), Clinical University Hospital Virgen de la Arrixaca, Murcia, Spain
Pablo Pelegrín
Bibliographic Information
Book Title: Purinergic Signaling
Book Subtitle: Methods and Protocols
Editors: Pablo Pelegrín
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-4939-9717-6
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020
Hardcover ISBN: 978-1-4939-9716-9Published: 24 October 2019
Softcover ISBN: 978-1-4939-9719-0Published: 24 October 2020
eBook ISBN: 978-1-4939-9717-6Published: 23 October 2019
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XII, 360
Number of Illustrations: 33 b/w illustrations, 49 illustrations in colour
Topics: Cell Biology