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Stem Cells and Aging

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kursad Turksen

    1. Ottawa, Canada

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2045)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xii
    Download chapter PDF

  2. Assessing Muscle Stem Cell Clonal Complexity During Aging

    • Matthew T. Tierney, Michael J. Stec, Alessandra Sacco
    Pages 1-11

  3. Simultaneous Isolation of Stem and Niche Cells of Skeletal Muscle: Applicability for Aging Studies

    • Eusebio Perdiguero, Victoria Moiseeva, Pura Muñoz-Cánoves
    Pages 13-23

  4. Isolation and Culture of Individual Myofibers and Their Adjacent Muscle Stem Cells from Aged and Adult Skeletal Muscle

    • Sören S. Hüttner, Hellen E. Ahrens, Manuel Schmidt, Henriette Henze, Marie Juliane Jung, Svenja C. Schüler et al.
    Pages 25-36Open Access

  5. Methods and Strategies for Procurement, Isolation, Characterization, and Assessment of Senescence of Human Mesenchymal Stem Cells from Adipose Tissue

    • Meenakshi Gaur, Marek Dobke, Victoria V. Lunyak
    Pages 37-92

  6. Quantifying Senescence-Associated Phenotypes in Primary Multipotent Mesenchymal Stromal Cell Cultures

    • Stéphanie Nadeau, Anastasia Cheng, Inés Colmegna, Francis Rodier
    Pages 93-105

  7. Adipogenic and Osteogenic Differentiation of In Vitro Aged Human Mesenchymal Stem Cells

    • Courtney R. Ogando, Gilda A. Barabino, Yueh-Hsun Kevin Yang
    Pages 107-117

  8. Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cell Isolation and Growth Kinetics Analysis

    • Klemen Čamernik, Janja Marc, Janja Zupan
    Pages 119-129

  9. Complete Assessment of Multilineage Differentiation Potential of Human Skeletal Muscle-Derived Mesenchymal Stem/Stromal Cells

    • Klemen Čamernik, Janja Zupan
    Pages 131-144

  10. Human Synovium-Derived Mesenchymal Stem Cells: Ex Vivo Analysis

    • Janja Zupan
    Pages 145-154

  11. 3D-Embedded Cell Cultures to Study Tendon Biology

    • Renate Gehwolf, Gabriel Spitzer, Andrea Wagner, Christine Lehner, Nadja Weissenbacher, Herbert Tempfer et al.
    Pages 155-165

  12. Targeted, Amplicon-Based, Next-Generation Sequencing to Detect Age-Related Clonal Hematopoiesis

    • Brooke Snetsinger, Christina K. Ferrone, Michael J. Rauh
    Pages 167-180

  13. Column-Free Method for Isolation and Culture of C-Kit Positive Stem Cells from Atrial Explants

    • Sherin Saheera, Renuka R. Nair
    Pages 181-186

  14. Histological Assessment of Cre-loxP Genetic Recombination in the Aging Subventricular Zone of Nestin-CreERT2/Rosa26YFP Mice

    • Saad Omais, Nour N. Halaby, Karl John Habashy, Carine Jaafar, Anthony T. Bejjani, Noël Ghanem
    Pages 187-199

  15. Infrared Spectroscopy and Imaging in Stem Cells and Aging Research

    • Ceren Aksoy, Feride Severcan
    Pages 201-215

  16. Use of U-STELA for Accurate Measurement of Extremely Short Telomeres

    • Nedime Serakinci, Huseyin Cagsin, Merdiye Mavis
    Pages 217-224

  17. Surface Antigen-Based Identification of In Vitro Expanded Skeletal Muscle-Derived Mesenchymal Stromal/Stem Cells Using Flow Cytometry

    • Klemen Čamernik, Janja Zupan
    Pages 225-233

  18. Analysis of Stem Cells and Their Activity in Human Skeletal Muscles by Immunohistochemistry

    • Rasmus Jentoft Boutrup
    Pages 235-244

  19. Methods for Detection of Autophagy in Mammalian Cells

    • Bindu Singh, Sangeeta Bhaskar
    Pages 245-258

  20. Metabolomic and Proteomic Analyses of Mouse Primordial Germ Cells

    • Yohei Hayashi, Yasuhisa Matsui
    Pages 259-269
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Keywords

About this book

This fully updated book collects protocols that reflect current investigations into how stem cell populations change during organismal aging. These methodologies are well-established and described in easy to follow fashion so as to be valuable for not only experts but novices in the ever-developing stem cell field. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Stem Cells and Aging: Methods and Protocols, Second Edition serves as an ideal guide for all researchers working in this very active area of study.

Editors and Affiliations

  • Ottawa, Canada

    Kursad Turksen

Bibliographic Information

  • Book Title: Stem Cells and Aging

  • Book Subtitle: Methods and Protocols

  • Editors: Kursad Turksen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9713-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9712-1Published: 05 September 2019

  • Softcover ISBN: 978-1-4939-9715-2Published: 05 September 2020

  • eBook ISBN: 978-1-4939-9713-8Published: 04 September 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XII, 354

  • Number of Illustrations: 3 b/w illustrations, 64 illustrations in colour

  • Topics: Stem Cells, Regenerative Medicine/Tissue Engineering, Cell Physiology

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