Skip to main content
SpringerLink
Log in
Book cover

Cerebrospinal Fluid (CSF) Proteomics

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Enrique Santamaría

    1. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Joaquín Fernández-Irigoyen

    1. Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2044)

This is a preview of subscription content, log in via an institution to check access.

Access this book

Log in via an institution
eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

Licence this eBook for your library

Institutional subscriptions

Table of contents (26 protocols)

  1. Front Matter

    Pages i-xv
    Download chapter PDF

  2. Introduction

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. The Human Brain Proteome Project: Biological and Technological Challenges

      • Joaquín Fernández-Irigoyen, Fernando Corrales, Enrique Santamaría
      Pages 3-23

  3. Cerebrospinal Fluid Sample Preparation Methods for Proteomic Workflows

    1. Front Matter

      Pages 25-25
      Download chapter PDF

    2. Guidelines for CSF Processing and Biobanking: Impact on the Identification and Development of Optimal CSF Protein Biomarkers

      • Yanaika S. Hok-A-Hin, Eline A. J. Willemse, Charlotte E. Teunissen, Marta Del Campo
      Pages 27-50

    3. Functional Analyses of Embryonic Cerebrospinal Fluid Proteins

      • Teresa Caprile, Francisco Lamus, María Isabel Alonso, Hernán Montecinos, Angel Gato
      Pages 51-60

    4. CSF Sample Preparation for Data-Independent Acquisition

      • Katalin Barkovits, Lars Tönges, Katrin Marcus
      Pages 61-67

    5. Sample Fractionation Techniques for CSF Peptide Spectral Library Generation

      • Sandra Pacharra, Katrin Marcus, Caroline May
      Pages 69-77

  4. Shotgun Cerebrospinal Fluid Proteomics

    1. Front Matter

      Pages 79-79
      Download chapter PDF

    2. Application of 2D-DIGE and iTRAQ Workflows to Analyze CSF in Gliomas

      • Aishwarya A. Rao, Kanika Mehta, Nikita Gahoi, Sanjeeva Srivastava
      Pages 81-110

    3. Peptidomic Workflow Applied to Cerebrospinal Fluid Analysis

      • Rustam H. Ziganshin, Sergey I. Kovalchuk, Igor V. Azarkin
      Pages 111-118

    4. Quantitative Evaluation of Different Protein Fractions of Cerebrospinal Fluid Using 18O Labeling

      • Ramona Birke, Eberhard Krause, Michael Schümann, Ingolf E. Blasig, Reiner F. Haseloff
      Pages 119-128

    5. A Versatile Workflow for Cerebrospinal Fluid Proteomic Analysis with Mass Spectrometry: A Matter of Choice between Deep Coverage and Sample Throughput

      • Charlotte Macron, Antonio Núñez Galindo, Ornella Cominetti, Loïc Dayon
      Pages 129-154

    6. Determination of Cerebrospinal Fluid Proteome Variations by Isobaric Labeling Coupled with Strong Cation-Exchange Chromatography and Tandem Mass Spectrometry

      • Mercedes Lachén-Montes, Andrea González-Morales, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 155-168

    7. SWATH Mass Spectrometry Applied to Cerebrospinal Fluid Differential Proteomics: Establishment of a Sample-Specific Method

      • Sandra I. Anjo, Cátia Santa, Bruno Manadas
      Pages 169-189

  5. Cerebrospinal Fluid Subproteomics

    1. Front Matter

      Pages 191-191
      Download chapter PDF

    2. Top-Down Proteomics Applied to Human Cerebrospinal Fluid

      • Marina Gay, Ester Sánchez-Jiménez, Laura Villarreal, Mar Vilanova, Romain Huguet, Gianluca Arauz-Garofalo et al.
      Pages 193-219

    3. Application of an Aptamer-Based Proteomics Assay (SOMAscan™) in Rat Cerebrospinal Fluid

      • Alba Simats, Laura Ramiro, Joan Montaner, Teresa García-Berrocoso
      Pages 221-231

    4. Monitoring the Cerebrospinal Fluid Cytokine Profile Using Membrane-Based Antibody Arrays

      • Andrea González-Morales, Mercedes Lachén-Montes, Joaquín Fernández-Irigoyen, Enrique Santamaría
      Pages 233-246

    5. Selective and Sensitive Mass Spectrometric Identification of Immune Complex Antigens in Cerebrospinal Fluid

      • Nozomi Aibara, Kaname Ohyama
      Pages 247-253
Back to top

Keywords

About this book

This volume focuses on protein analysis, including a wide range of the use of mass spectrometry and other protein methods within neurobiological disciplines. Chapters cover topics such as cerebrospinal fluid (CSF) processing and biobanking; label-free quantitative proteomics; SWATH; top-down proteomics; and experimental strategies based on other –omics applied to CSF metabolome, lipidome, and microRNAome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Cerebrospinal Fluid (CSF) Proteomics: Methods and Protocols is a valuable resource for graduate students and post-doctoral fellows interested in learning more about CSF proteotyping. It is also useful to established researchers seeking furtherinsight into this growing field. 


Editors and Affiliations

  • Proteomics Unit, Clinical Neuroproteomics Laboratory, Navarrabiomed, Complejo Hospitalario de Navarra (CHN), Universidad Pública de Navarra (UPNA), IdiSNA, Proteored-ISCIII, Pamplona, Spain

    Enrique Santamaría, Joaquín Fernández-Irigoyen

Bibliographic Information

  • Book Title: Cerebrospinal Fluid (CSF) Proteomics

  • Book Subtitle: Methods and Protocols

  • Editors: Enrique Santamaría, Joaquín Fernández-Irigoyen

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9706-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9705-3Published: 21 August 2019

  • Softcover ISBN: 978-1-4939-9708-4Published: 21 August 2020

  • eBook ISBN: 978-1-4939-9706-0Published: 20 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XV, 557

  • Number of Illustrations: 3 b/w illustrations, 164 illustrations in colour

  • Topics: Neurosciences, Neurobiology, Proteomics

Publish with us

Policies and ethics

Back to top

Search

Navigation