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Multiphoton Microscopy

  • Book
  • © 2019

Overview

Editors:
  1. Espen Hartveit

    1. Department of Biomedicine, University of Bergen, Bergen, Norway

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains keynotes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 148)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii
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  2. Building a Two-Photon Microscope Is Easy

    • Spencer LaVere Smith
    Pages 1-16

  3. Using Multiphoton Imaging for Targeted Electrophysiological Recording and Live Cell Imaging of Fluorescently Labeled Neurons from Isolated Retinas

    • Qiang Chen, Wei Wei
    Pages 17-31

  4. Two-Photon Neurotransmitter Uncaging for the Study of Dendritic Integration

    • Alexandra Tran-Van-Minh, Nelson Rebola, Andreas Hoehne, David A. DiGregorio
    Pages 33-64

  5. Two-Photon Glutamate Uncaging to Study Structural and Functional Plasticity of Dendritic Spines

    • Ivar S. Stein, Travis C. Hill, Won Chan Oh, Laxmi K. Parajuli, Karen Zito
    Pages 65-85

  6. Two-Photon Fluorescence Imaging of Visually Evoked Glutamate Release Using iGluSnFR in the Mouse Visual System

    • Bart G. Borghuis
    Pages 87-109

  7. Imaging Neuronal Signal Transduction Using Multiphoton FRET-FLIM

    • Paul R. Evans, Long Yan, Ryohei Yasuda
    Pages 111-130

  8. Combining Multiphoton Excitation Microscopy with Fast Microiontophoresis to Investigate Neuronal Signaling

    • Espen Hartveit, Margaret Lin Veruki
    Pages 131-159

  9. Multiphoton Excitation Microscopy for the Reconstruction and Analysis of Single Neuron Morphology

    • Espen Hartveit, Bas-Jan Zandt, Margaret Lin Veruki
    Pages 161-194

  10. Serial Multiphoton Tomography and Analysis of Volumetric Images of the Mouse Brain

    • Denise M. O. Ramirez, Apoorva D. Ajay, Mark P. Goldberg, Julian P. Meeks
    Pages 195-224

  11. Studying a Light Sensor with Light: Multiphoton Imaging in the Retina

    • Thomas Euler, Katrin Franke, Tom Baden
    Pages 225-250

  12. Multiphoton Ca2+ Imaging of Astrocytes with Genetically Encoded Indicators Delivered by a Viral Approach

    • Rune Enger, Rolf Sprengel, Erlend A. Nagelhus, Wannan Tang
    Pages 251-277

  13. Two-Photon Imaging of Dendritic Calcium Dynamics In Vivo

    • Lucy M. Palmer
    Pages 279-295

  14. Voltage Imaging with ANNINE Dyes and Two-Photon Microscopy

    • Christopher J. Roome, Bernd Kuhn
    Pages 297-334

  15. High-Speed, Random-Access Multiphoton Microscopy for Monitoring Synaptic and Neuronal Activity in 3D in Behaving Animals

    • Mate Marosi, Gergely Szalay, Gergely Katona, Balázs Rózsa
    Pages 335-362

  16. Back Matter

    Pages 363-364
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Keywords

About this book

This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.


Comprehensive and thorough, Multiphoton Microscopy is a valuable resource for both expert and novice researchers interested in expanding their knowledge and research in this rapidly developing field.



Reviews

“I recommend this exciting book, replete with full-color images, because of its comprehensive, tested protocols for the study of live neural tissue at the cellular and the subcellular levels.” (Barry R. Masters, Optics & Photonics News, osa-opn.org, March 5, 2020)

Editors and Affiliations

  • Department of Biomedicine, University of Bergen, Bergen, Norway

    Espen Hartveit

Bibliographic Information

  • Book Title: Multiphoton Microscopy

  • Editors: Espen Hartveit

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-9702-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9701-5Published: 01 November 2019

  • Softcover ISBN: 978-1-4939-9704-6Published: 01 November 2020

  • eBook ISBN: 978-1-4939-9702-2Published: 31 October 2019

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 364

  • Number of Illustrations: 4 b/w illustrations, 82 illustrations in colour

  • Topics: Neurosciences, Biological Microscopy

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