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ADAMTS Proteases

Methods and Protocols

  • Book
  • © 2020

Overview

Editors:
  1. Suneel S. Apte

    1. Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, USA

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2043)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-x
    Download chapter PDF

  2. ADAMTS Proteins: Concepts, Challenges, and Prospects

    • Suneel S. Apte
    Pages 1-12

  3. Inhibition of ADAMTS1 Expression by Lentiviral CRISPR/Cas9 Gene Editing Technology

    • Carlos Peris-Torres, Orlando Serrano, María del Carmen Plaza-Calonge, Juan Carlos Rodríguez-Manzaneque
    Pages 13-24

  4. Analyzing the Effects of O-Fucosylation on Secretion of ADAMTS Proteins Using Cell-Based Assays

    • Steven John Berardinelli, Robert S. Haltiwanger
    Pages 25-43

  5. Challenges and Solutions for Purification of ADAMTS Proteases: An Overview

    • Alain C. Colige
    Pages 45-53

  6. Purification of Native or Recombinant ADAMTS2, and Procollagen I Cleavage Assay

    • Alain C. Colige
    Pages 55-62

  7. ADAMTS7: Recombinant Protein Expression and Purification

    • Rens de Groot
    Pages 63-73

  8. Purification and Activity Determination of ADAMTS-4 and ADAMTS-5 and Their Domain Deleted Mutants

    • Milan M. Fowkes, Ngee H. Lim
    Pages 75-91

  9. Expression and Preparation of Recombinant Reelin and ADAMTS-3 Proteins

    • Takao Kohno, Himari Ogino, Yuko Yamakage, Mitsuharu Hattori
    Pages 93-104

  10. Assay for Reelin-Cleaving Activity of ADAMTS and Detection of Reelin and Its Fragments in the Brain

    • Himari Ogino, Yuko Yamakage, Mihoshi B. Yamashita, Takao Kohno, Mitsuharu Hattori
    Pages 105-111

  11. Chemical Modification of Proteoglycanases with Biotin

    • Salvatore Santamaria
    Pages 113-123

  12. Analysis of Aggrecanase Activity Using Neoepitope Antibodies

    • Salvatore Santamaria, Kazuhiro Yamamoto
    Pages 125-136

  13. Molecular Cloning, Lentiviral Transduction, and Expression of Recombinant ADAMTSL2 and ADAMTSL4

    • Mukti Singh, Stuart A. Cain, Clair Baldock
    Pages 137-155

  14. Purification of Recombinant ADAMTSL2

    • Mukti Singh, Clair Baldock
    Pages 157-172

  15. Expression Analysis by RNAscope™ In Situ Hybridization

    • Timothy J. Mead, Suneel S. Apte
    Pages 173-178

  16. Analysis of ADAMTS Effects on Cell Adhesion and Migration

    • Jordi Lambert, Dylan R. Edwards
    Pages 179-193

  17. Cell-Based Interaction Analysis of ADAMTS Proteases and ADAMTS-Like Proteins with Fibrillin Microfibrils

    • Dirk Hubmacher
    Pages 195-206

  18. Alizarin Red and Alcian Blue Preparations to Visualize the Skeleton

    • Timothy J. Mead
    Pages 207-212

  19. Biophysical Techniques to Analyze Elastic Tissue Extracellular Matrix Proteins Interacting with ADAMTS Proteins

    • Valentin Nelea, Dieter P. Reinhardt
    Pages 213-235

  20. Quantification of Extracellular Matrix Fiber Systems Related to ADAMTS Proteins

    • Rong-Mo Zhang, Heena Kumra, Dieter P. Reinhardt
    Pages 237-250
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Keywords

About this book

This volume provides a variety of methods used to analyze ADAMTS proteases and ADAMTS-like proteins, including their structure, substrate profile, tissue and cell distribution, post-translational modification and biological pathways. Chapters discuss genetic, cell biology, biochemical and proteomics techniques applicable to the analysis of all the members of the ADAMTS superfamily. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 
Cutting-edge and thorough, ADAMTS Proteases: Methods and Protocols is a valuable tool for researchers to study both simple and sophisticated methodologies that are often difficult to locate in primary research literature. 


Editors and Affiliations

  • Department of Biomedical Engineering-ND20, Cleveland Clinic Lerner Research Institute, Cleveland, USA

    Suneel S. Apte

Bibliographic Information

  • Book Title: ADAMTS Proteases

  • Book Subtitle: Methods and Protocols

  • Editors: Suneel S. Apte

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9698-8

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2020

  • Hardcover ISBN: 978-1-4939-9697-1Published: 29 August 2019

  • Softcover ISBN: 978-1-4939-9700-8Published: 29 August 2020

  • eBook ISBN: 978-1-4939-9698-8Published: 28 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 299

  • Number of Illustrations: 12 b/w illustrations, 47 illustrations in colour

  • Topics: Protein Science, Proteomics

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