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Imaging Gene Expression

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Yaron Shav-Tal

    1. The Mina & Everard Goodman, Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan, Israel

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2038)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xiii
    Download chapter PDF

  2. Imaging RNAs and Gene Expression in Cells and Organisms

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Imaging Single mRNA Molecules in Mammalian Cells Using an Optimized MS2-MCP System

      • Maria Vera, Evelina Tutucci, Robert H. Singer
      Pages 3-20

    3. Live-Cell Imaging of Long Noncoding RNAs Using Molecular Beacons

      • Yachen Ying, Shiqi Mao, Christopher J. Krueger, Antony K. Chen
      Pages 21-33

    4. Real-Time Observation of Localization and Expression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene

      • Hiroshi Ochiai
      Pages 35-45

    5. Visualization of Single mRNAs in Live Neurons

      • Jae Youn Shim, Byung Hun Lee, Hye Yoon Park
      Pages 47-61

    6. Lighting Up Gene Activation in Living Drosophila Embryos

      • Carola Fernandez, Mounia Lagha
      Pages 63-74

    7. Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis

      • Franka Voigt, Jan Eglinger, Jeffrey A. Chao
      Pages 75-88

    8. Imaging rRNA Methylation in Bacteria by MR-FISH

      • Kristina A. Ganzinger, Martin R. Challand, James Spencer, David Klenerman, Rohan T. Ranasinghe
      Pages 89-107

    9. Detection of mRNA Transfer Between Mammalian Cells in Coculture by Single-Molecule Fluorescent In Situ Hybridization (smFISH)

      • Gal Haimovich, Jeffrey E. Gerst
      Pages 109-129

    10. Live-Cell Imaging of mRNP–NPC Interactions in Budding Yeast

      • Azra Lari, Farzin Farzam, Pierre Bensidoun, Marlene Oeffinger, Daniel Zenklusen, David Grunwald et al.
      Pages 131-150

    11. Detection of mRNAs Anchored to the Nuclear Envelope During Export Inhibition in Living Cells

      • Rakefet Ben-Yishay, Yaron Shav-Tal
      Pages 151-163

  3. Imaging Chromosomes, Genes, and Transcription Factors

    1. Front Matter

      Pages 165-165
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    2. Programmable Chromosome Painting with Oligopaints

      • Son C. Nguyen, Eric F. Joyce
      Pages 167-180

    3. Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices

      • Roopali Pradhan, Kundan Sengupta
      Pages 181-197

    4. Targeted Manipulation/Repositioning of Subcellular Structures and Molecules

      • Kathrin S. Heinz, M. Cristina Cardoso
      Pages 199-208

    5. Visualization of Endogenous Transcription Factors in Single Cells Using an Antibody Electroporation-Based Imaging Approach

      • Sascha Conic, Dominique Desplancq, Alexia Ferrand, Nacho Molina, Etienne Weiss, László Tora
      Pages 209-221

    6. Monitoring Transcription Factor Oligomerization in Single Living Cells by Number and Brightness Analysis

      • Eugenia Cammarota, Davide Mazza
      Pages 223-237

    7. Visualizing Transcription Factor Binding on Mitotic Chromosomes Using Single-Molecule Live-Cell Imaging

      • James Z. J. Kwan, Thomas F. Nguyen, Sheila S. Teves
      Pages 239-250

    8. Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells

      • Jorge Trojanowski, Anne Rademacher, Fabian Erdel, Karsten Rippe
      Pages 251-270
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Keywords

About this book

This volume explores the latest updates on microscopy approaches and techniques used by scientists studying in the field of gene expression imaging. These updates cover the technical design of the experiments and the expected outcomes. The chapters in this book are divided into two parts: Part One looks at the output of a gene, in particular the RNA molecules that are copied from the gene itself; and Part Two focuses on chromosomes, chromatin, and factors that bind DNA. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
 
Cutting-edge and thorough, Imaging Gene Expression: Methods and Protocols, Second Edition is a valuable resource for any researcher interested in learning more about this evolving and important field. 


Editors and Affiliations

  • The Mina & Everard Goodman, Faculty of Life Sciences and the Institute of Nanotechnology and Advanced Materials, Bar-Ilan University, Ramat Gan, Israel

    Yaron Shav-Tal

Bibliographic Information

  • Book Title: Imaging Gene Expression

  • Book Subtitle: Methods and Protocols

  • Editors: Yaron Shav-Tal

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9674-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9673-5Published: 13 August 2019

  • Softcover ISBN: 978-1-4939-9676-6Published: 13 August 2020

  • eBook ISBN: 978-1-4939-9674-2Published: 12 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIII, 272

  • Number of Illustrations: 1 b/w illustrations, 46 illustrations in colour

  • Topics: Gene Expression, Biological Microscopy

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