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  • © 2019

Oligonucleotide-Based Therapies

Methods and Protocols

Editors:

  1. Olof Gissberg

    1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

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    You can also search for this editor in PubMed   Google Scholar

  2. Rula Zain

    1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  3. Karin E. Lundin

    1. Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2036)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    PDF

  2. Reviews

    1. Front Matter

      Pages 1-1
      PDF

    2. Chemical Development of Therapeutic Oligonucleotides

      • Karin E. Lundin, Olof Gissberg, C. I. Edvard Smith, Rula Zain
      Pages 3-16

    3. Tissue-Specific Delivery of Oligonucleotides

      • Xin Xia, Nicolette Pollock, Jiehua Zhou, John Rossi
      Pages 17-50

  3. Protocols and Strategies

    1. Front Matter

      Pages 51-51
      PDF

    2. Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot

      • Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko, Caroline Palm-Apergi
      Pages 53-72

    4. Assessing Oligonucleotide Binding to Double-Stranded DNA

      • Negin Mozafari, Tea Umek
      Pages 91-112

    5. Modeling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy

      • Lennart Nilsson, Alessandra Villa
      Pages 113-126

    7. Click-Shielded and Targeted Lipopolyplexes

      • Philipp Michael Klein, Ernst Wagner
      Pages 141-164

    10. Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)

      • Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, Sterghios A. Moschos
      Pages 187-203Open Access

    11. Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury

      • Pedro M. D. Moreno, Teresa Rodrigues, Marília Torrado, Isabel F. Amaral, Ana P. Pêgo
      Pages 205-219

    12. Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy

      • Suzan M. Hammond, Frank Abendroth, Michael J. Gait, Matthew J. A. Wood
      Pages 221-236

    14. In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides

      • Sabine Sewing, Tanja Minz, Franziska Boess
      Pages 249-259
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About this book

This book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics. Covering topics from the study of antisense and anti-gene effects to oligonucleotides in the context of drug discovery and development, the volume explores a wide-ranging and useful spectrum of methods and protocols needed to take full advantage of therapeutic applications involving ONs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Oligonucleotide-Based Therapies: Methods and Protocols aims to be a great aid in the laboratory as well as an ideal reference guide when designing antisense and anti-gene oligonucleotides for therapeutic applications.
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Keywords

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Editors and Affiliations

  • Center for Advanced Therapies, Department of Laboratory Medicine, Karolinska Institutet, Stockholm, Sweden

    Olof Gissberg, Rula Zain, Karin E. Lundin

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Bibliographic Information

  • Book Title: Oligonucleotide-Based Therapies

  • Book Subtitle: Methods and Protocols

  • Editors: Olof Gissberg, Rula Zain, Karin E. Lundin

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9670-4

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9669-8Published: 14 August 2019

  • Softcover ISBN: 978-1-4939-9672-8Published: 15 August 2020

  • eBook ISBN: 978-1-4939-9670-4Published: 13 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 344

  • Number of Illustrations: 23 b/w illustrations, 46 illustrations in colour

  • Topics: Laboratory Medicine, Human Genetics, Pharmacology/Toxicology

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Access via your institution

Buy it now

eBook USD 99.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 129.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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