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Microglia

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Olga Garaschuk

    1. Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

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  2. Alexei Verkhratsky

    1. Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2034)

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Table of contents (24 protocols)

  1. Front Matter

    Pages i-xii
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  2. Microglia in Health and Disease

    1. Front Matter

      Pages 1-1
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    2. Microglia: The Neural Cells of Nonneural Origin

      • Olga Garaschuk, Alexei Verkhratsky
      Pages 3-11

    3. Morphology of Microglia Across Contexts of Health and Disease

      • Julie C. Savage, Micaël Carrier, Marie-Ève Tremblay
      Pages 13-26

    4. Physiology of Microglia

      • Olga Garaschuk, Alexei Verkhratsky
      Pages 27-40

    5. Microglia Reactivity: Heterogeneous Pathological Phenotypes

      • Hélène Hirbec, François Rassendren, Etienne Audinat
      Pages 41-55

    6. Microglia in Neurodegenerative Disorders

      • Darío Tejera, Michael T. Heneka
      Pages 57-67

    7. Physiological Implications of Microglia–Synapse Interactions

      • Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, Junichi Nabekura
      Pages 69-80

  3. In Vitro Analyses of Microglia

    1. Front Matter

      Pages 81-81
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    2. Ultrastructural Analyses of Microglial Interactions with Synapses

      • Marie-Ève Tremblay, Ania K. Majewska
      Pages 83-95

    3. Visualizing Dark Microglia

      • Marie-Kim St-Pierre, Maude Bordeleau, Marie-Ève Tremblay
      Pages 97-110

    4. Electrophysiological Investigation of Microglia

      • Elena Avignone, Giampaolo Milior, Isabelle Arnoux, Etienne Audinat
      Pages 111-125

    5. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia

      • Annette Masuch, Knut Biber
      Pages 127-147

    6. Assessment of Parasite–Microglia Interactions In Vitro

      • Katherine Figarella, Nestor L. Uzcategui, Michael Duszenko
      Pages 149-161

  4. Studying Microglia In Vivo

    1. Front Matter

      Pages 163-163
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    2. In Vivo Visualization of Microglia Using Tomato Lectin

      • Bianca Brawek, Maria Olmedillas del Moral, Olga Garaschuk
      Pages 165-175

    3. Long-Term In Vivo Imaging of Individual Microglial Cells

      • Angelos A. Skodras, Jasmin K. Hefendehl, Jonas J. Neher
      Pages 177-189

    4. Analysis of Signaling Mechanisms Regulating Microglial Process Movement

      • Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, Christian Madry
      Pages 191-205

    5. Measuring Microglial Turnover in the Adult Brain

      • Diego Gomez-Nicola, Gemma L. Fryatt, Katharine E. Askew
      Pages 207-215

    6. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor

      • Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, Ari Waisman
      Pages 217-230
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Keywords

About this book

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.

Editors and Affiliations

  • Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

    Olga Garaschuk

  • Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

    Alexei Verkhratsky

Bibliographic Information

  • Book Title: Microglia

  • Book Subtitle: Methods and Protocols

  • Editors: Olga Garaschuk, Alexei Verkhratsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9658-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9657-5Published: 08 August 2019

  • Softcover ISBN: 978-1-4939-9660-5Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9658-2Published: 07 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 340

  • Number of Illustrations: 59 b/w illustrations, 6 illustrations in colour

  • Topics: Neurosciences, Immunology

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