Microglia

1. Front Matter

   Pages i-xii

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2. Microglia in Health and Disease

   1. Front Matter

      Pages 1-1

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   2. Microglia: The Neural Cells of Nonneural Origin

      • Olga Garaschuk, Alexei Verkhratsky

      Pages 3-11

   3. Morphology of Microglia Across Contexts of Health and Disease

      • Julie C. Savage, Micaël Carrier, Marie-Ève Tremblay

      Pages 13-26

   4. Physiology of Microglia

      • Olga Garaschuk, Alexei Verkhratsky

      Pages 27-40

   5. Microglia Reactivity: Heterogeneous Pathological Phenotypes

      • Hélène Hirbec, François Rassendren, Etienne Audinat

      Pages 41-55

   6. Microglia in Neurodegenerative Disorders

      • Darío Tejera, Michael T. Heneka

      Pages 57-67

   7. Physiological Implications of Microglia–Synapse Interactions

      • Hiroaki Wake, Hiroshi Horiuchi, Daisuke Kato, Andrew J. Moorhouse, Junichi Nabekura

      Pages 69-80

3. In Vitro Analyses of Microglia

   1. Front Matter

      Pages 81-81

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   2. Ultrastructural Analyses of Microglial Interactions with Synapses

      • Marie-Ève Tremblay, Ania K. Majewska

      Pages 83-95

   3. Visualizing Dark Microglia

      • Marie-Kim St-Pierre, Maude Bordeleau, Marie-Ève Tremblay

      Pages 97-110

   4. Electrophysiological Investigation of Microglia

      • Elena Avignone, Giampaolo Milior, Isabelle Arnoux, Etienne Audinat

      Pages 111-125

   5. Replenishment of Organotypic Hippocampal Slice Cultures with Neonatal or Adult Microglia

      • Annette Masuch, Knut Biber

      Pages 127-147

   6. Assessment of Parasite–Microglia Interactions In Vitro

      • Katherine Figarella, Nestor L. Uzcategui, Michael Duszenko

      Pages 149-161

4. Studying Microglia In Vivo

   1. Front Matter

      Pages 163-163

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   2. In Vivo Visualization of Microglia Using Tomato Lectin

      • Bianca Brawek, Maria Olmedillas del Moral, Olga Garaschuk

      Pages 165-175

   3. Long-Term In Vivo Imaging of Individual Microglial Cells

      • Angelos A. Skodras, Jasmin K. Hefendehl, Jonas J. Neher

      Pages 177-189

   4. Analysis of Signaling Mechanisms Regulating Microglial Process Movement

      • Vasiliki Kyrargyri, David Attwell, Renaud Blaise Jolivet, Christian Madry

      Pages 191-205

   5. Measuring Microglial Turnover in the Adult Brain

      • Diego Gomez-Nicola, Gemma L. Fryatt, Katharine E. Askew

      Pages 207-215

   6. Novel Microglia Depletion Systems: A Genetic Approach Utilizing Conditional Diphtheria Toxin Receptor Expression and a Pharmacological Model Based on the Blocking of Macrophage Colony-Stimulating Factor 1 Receptor

      • Maja Kitic, Peter See, Julia Bruttger, Florent Ginhoux, Ari Waisman

      Pages 217-230

This book presents a comprehensive toolkit of versatile techniques for studying microglia under different experimental settings along with a brief summary of knowledge, accumulated in microglial research over the last decades. Beginning with recently discovered roles of microglia in health and disease, the volume continues by covering in vitro analyses of microglia, in vivo studies, and “omics” analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and cutting-edge, Microglia: Methods and Protocols serves as a vital guide to these important cells and an inspiration for scientists interested in expanding our knowledge of their role in the nervous system.

• Functional brain networks
• Immune mediators
• Microglial research
• Synaptic contacts
• Cognitive ability
• Brain immunity

• Department of Neurophysiology, Institute of Physiology, Eberhard Karls University of Tübingen, Tübingen, Germany

  Olga Garaschuk

• Faculty of Biology, Medicine and Health The University of Manchester, , Center for Basic and Translational Neuroscience, Faculty of Health and Medical Sciences University of Copenhagen Copenhagen, Denmark, Achucarro Center for Neuroscience IKERBASQUE Basque Foundation for Science Bilbao, Spain, Manchester, UK

  Alexei Verkhratsky

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