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Genotoxicity Assessment

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Alok Dhawan

    1. CSIR—Indian Institute of Toxicology Research, Lucknow, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  2. Mahima Bajpayee

    1. New Delhi, India

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2031)

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Table of contents (20 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Gene Mutation Assays

    1. Front Matter

      Pages 1-1
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    2. In Vitro Mouse Lymphoma Cell (L5178Y Tk+/− −3.7.2.C) Forward Mutation Assay

      • Melissa R. Schisler, Martha M. Moore, B. Bhaskar Gollapudi
      Pages 3-28

    3. Erythrocyte-Based Pig-a Gene Mutation Assay

      • Jeffrey C. Bemis, Nikki E. Hall, Stephen D. Dertinger
      Pages 29-57

    4. Detection of In Vivo Mutation in the Hprt and Pig-a Genes of Rat Lymphocytes

      • Vasily N. Dobrovolsky, Joseph G. Shaddock, Roberta A. Mittelstaedt, Daishiro Miura, Robert H. Heflich
      Pages 59-75

  3. Assays for Chromosomal Abnormalities

    1. Front Matter

      Pages 77-77
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    2. In Vitro Cytogenetic Assays: Chromosomal Aberrations and Micronucleus Tests

      • Pasquale Mosesso, Serena Cinelli
      Pages 79-104

    3. Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry

      • Antonella Testa, Valentina Palma, Clarice Patrono
      Pages 105-119

    4. Chromosomal Aberration Test in Human Lymphocytes

      • Christian Johannes, Guenter Obe
      Pages 121-134

    5. In Vivo Micronucleus Assay in Mouse Bone Marrow

      • Abhishek Kumar Jain, Alok Kumar Pandey
      Pages 135-146

    6. Micronucleus Cytome Assays in Human Lymphocytes and Buccal Cells

      • Claudia Bolognesi, Michael Fenech
      Pages 147-163

    7. Micronucleus Analysis by Flow Cytometry

      • Azeddine Elhajouji, Pascal Stadelmann
      Pages 165-193

    8. Hen’s Egg Test for Micronucleus Induction (HET-MN)

      • Kerstin Reisinger, Eva Dony, Thorsten Wolf, Katrin Maul
      Pages 195-208

    9. Comparative Genomic Hybridization (CGH) in Genotoxicology

      • Adi Baumgartner, Veronika Hartleb, Jim D. Taylor
      Pages 209-234

  4. Tests for Primary DNA Damage

    1. Front Matter

      Pages 235-235
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    2. The Comet Assay: Assessment of In Vitro and In Vivo DNA Damage

      • Mahima Bajpayee, Ashutosh Kumar, Alok Dhawan
      Pages 237-257

    3. The Comet Assay in Human Biomonitoring

      • Diana Anderson, Alok Dhawan, Julian Laubenthal
      Pages 259-274

    4. The Comet Assay in Marine Animals

      • Patrizia Guidi, Brett P. Lyons, Giada Frenzilli
      Pages 275-286

    5. Multicolor Laser Scanning Confocal Immunofluorescence Microscopy of DNA Damage Response Biomarkers

      • Julian Laubenthal, Michal R. Gdula, Alok Dhawan, Diana Anderson
      Pages 287-300

    6. Detection of DNA Double-Strand Breaks Using Pulsed-Field Gel Electrophoresis

      • Vyom Sharma, Vishwa Mohan
      Pages 301-311
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Keywords

About this book

This current edition explores new tests for genotoxicity testing, along with other well-known techniques. This will further help in our understanding of the genotoxic effects of chemicals. The book has different sections dealing with various assays for gene mutation, chromosomal abnormalities, primary DNA damage, etc. It also delves into plant models, animals and their alternates, as well as in silico approaches for genetic toxicology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls. 

Authoritative and up-to-date, Genotoxicity Assessment: Methods and Protocols, Second Edition serves as a highly useful and ready resource for research students and scientists working in regulatory toxicology as well as biomedical, biochemical, and pharmaceutical sciences.

Editors and Affiliations

  • CSIR—Indian Institute of Toxicology Research, Lucknow, India

    Alok Dhawan

  • New Delhi, India

    Mahima Bajpayee

Bibliographic Information

  • Book Title: Genotoxicity Assessment

  • Book Subtitle: Methods and Protocols

  • Editors: Alok Dhawan, Mahima Bajpayee

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9646-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9645-2Published: 02 September 2019

  • Softcover ISBN: 978-1-4939-9648-3Published: 02 September 2020

  • eBook ISBN: 978-1-4939-9646-9Published: 31 August 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XI, 378

  • Number of Illustrations: 37 b/w illustrations, 57 illustrations in colour

  • Topics: Pharmacology/Toxicology, Human Genetics, Ecotoxicology

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