Amino Acid Analysis

1. Front Matter

   Pages i-xvii

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2. Ultrasensitive Quantification of Recombinant Proteins Using AAA-MS

   • Mathilde Louwagie, Sylvie Kieffer-Jaquinod, Virginie Brun

   Pages 1-10

3. Absolute Quantitation of Proteins by Acid Hydrolysis Combined with Amino Acid Detection by Mass Spectrometry

   • Olga A. Mirgorodskaya, Roman Körner, Yuri P. Kozmin, Peter Roepstorff

   Pages 11-16

4. Amino Acid Analysis by Means of MALDI TOF Mass Spectrometry or MALDI TOF/TOF Tandem Mass Spectrometry

   • Natalia V. Gogichaeva, Michail A. Alterman

   Pages 17-31

5. Multiple Reaction Monitoring for the Accurate Quantification of Amino Acids: Using Hydroxyproline to Estimate Collagen Content

   • Michelle L. Colgrave, Peter G. Allingham, Kerri Tyrrell, Alun Jones

   Pages 33-45

6. Analysis of Hydroxyproline in Collagen Hydrolysates

   • Tobias Langrock, Ralf Hoffmann

   Pages 47-56

7. Quantification of Isotopologues of Amino Acids by Multiplexed Stable Isotope-Resolved Metabolomics Using Ultrahigh-Resolution Mass Spectrometry Coupled with Direct Infusion

   • Ye Yang, Teresa W.-M. Fan, Andrew N. Lane, Richard M. Higashi

   Pages 57-68

8. Preparative HPLC Separation of Underivatized Amino Acids for Isotopic Analysis

   • Jennifer A. Tripp, Thibaut Devièse, James S. O. McCullagh

   Pages 69-83

9. Quantitative Analysis of Underivatized Amino Acids by Liquid Chromatography-Tandem Mass Spectrometry

   • Justin Mak, Tina M. Cowan, Anthony Le

   Pages 85-109

10. Amino Acid Analysis by Hydrophilic Interaction Chromatography Coupled with Isotope Dilution Mass Spectrometry

    • Megumi Kato, Akiko Takatsu

    Pages 111-118

11. The EZ:Faast Family of Amino Acid Analysis Kits: Application of the GC-FID Kit for Rapid Determination of Plasma Tryptophan and Other Amino Acids

    • Abdulla A.-B. Badawy

    Pages 119-130

12. Analysis of Tryptophan and Its Metabolites by High-Performance Liquid Chromatography

    • Zhaolai Dai, Shiqiang Sun, Hui Chen, Moyan Liu, Lianhua Zhang, Zhenlong Wu et al.

    Pages 131-142

13. Isotope Dilution Liquid Chromatography-Tandem Mass Spectrometry for Quantitative Amino Acid Analysis

    • David M. Bunk, Mark S. Lowenthal

    Pages 143-151

14. Quantification of Underivatized Amino Acids on Dry Blood Spot, Plasma, and Urine by HPLC-ESI-MS/MS

    • Giuseppe Giordano, Antonina Gucciardi, Paola Pirillo, Mauro Naturale

    Pages 153-172

15. Amino Acid Analysis in Physiological Samples by GC-MS with Propyl Chloroformate Derivatization and iTRAQ-LC-MS/MS

    • Katja Dettmer, Axel P. Stevens, Stephan R. Fagerer, Hannelore Kaspar, Peter J. Oefner

    Pages 173-190

16. Combination of an AccQ•Tag-Ultra-Performance Liquid Chromatographic Method with Tandem Mass Spectrometry for the Analysis of Amino Acids

    • Carolina Salazar, Jenny M. Armenta, Diego F. Cortés, Vladimir Shulaev

    Pages 191-206

17. Methods for Absolute Quantification of Human Plasma Free Amino Acids by High-Performance Liquid Chromatography/Electrospray Ionization Mass Spectrometry Using Precolumn Derivatization

    • Akira Nakayama, Akira Imaizumi, Hiroo Yoshida

    Pages 207-218

18. Analysis and Enantioseparation of Amino Acids by Liquid Chromatography

    • Poonam Malik, Ravi Bhushan

    Pages 219-236

19. Heptafluorobutyl Chloroformate-Based Sample Preparation Protocol for Nonchiral and Chiral Amino Acid Analysis by Gas Chromatography-Mass Spectrometry

    • Petr Šimek, Petr Hušek, Helena Zahradníčková

    Pages 237-251

20. High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization

    • Yosuke Nakano, Moyu Taniguchi, Yutaka Umakoshi, Daisuke Watai, Eiichiro Fukusaki

    Pages 253-261

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method; another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols, Second Edition presents a broad spectrum of all available methods allowing readers to choose the method that best suits their particular laboratory set-up and analytical needs. In this volume, readers can find chapters describing general, as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry, as well as in food analysis, microbiology, marine biology, drug metabolism, even archaeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.



Authoritative and accessible, Amino Acid Analysis: Methods and Protocols, Second Edition provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

• Metabolic Phenotypes
• RP-HPLC
• Mass Spectrometry
• Phenylthiocarbamyl Amino Acid Adducts
• Liquid Chromatography

• Office of Policy for Pharmaceutical Quality Office of Pharmaceutical Quality, Center for Drug Evaluation and Research US FDA, Silver Spring, USA

  Michail A. Alterman

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