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Antiviral Resistance in Plants

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Kappei Kobayashi

    1. Faculty of Agriculture, Ehime University, Matsuyama, Japan

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    You can also search for this editor in PubMed   Google Scholar

  2. Masamichi Nishiguchi

    1. Faculty of Agriculture, Ehime University, Matsuyama, Japan

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2028)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Identification and Functional Analysis of NB-LRR-Type Virus Resistance Genes: Overview and Functional Analysis of Candidate Genes

    • Reiko Tomita, Ken-Taro Sekine, Chika Tateda, Kappei Kobayashi
    Pages 1-10

  3. Insertion of Epitope Tag into NB-LRR Class Plant Virus Resistance Protein

    • Kappei Kobayashi, Reiko Tomita, Ken-Taro Sekine
    Pages 11-25

  4. Reverse Genetic Analysis of Antiviral Resistance Signaling and the Resistance Mechanism in Arabidopsis thaliana

    • Yukiyo Sato, Hideki Takahashi
    Pages 27-84

  5. Analysis of Antiviral Resistance Signaling Pathways by Virus-Induced Gene Silencing in Nicotiana benthamiana

    • Masayoshi Hashimoto, Yasuyuki Yamaji, Ken Komatsu
    Pages 85-95

  6. Random Mutagenesis of Virus Gene for the Experimental Evaluation of the Durability of NB-LRR Class Plant Virus Resistance Gene

    • Kengo Idehara, Reiko Tomita, Ken-Taro Sekine, Masamichi Nishiguchi, Kappei Kobayashi
    Pages 97-113

  7. A Cell-Free Replication System for Positive-Strand RNA Viruses for Identification and Characterization of Plant Resistance Gene Products

    • Kazuhiro Ishibashi, Masayuki Ishikawa
    Pages 115-122

  8. Plant Protein-Mediated Inhibition of Virus Cell-to-Cell Movement: Far-Western Screening and Biological Analysis of a Plant Protein Interacting with a Viral Movement Protein

    • Nobumitsu Sasaki, Yasuhiko Matsushita, Hiroshi Nyunoya
    Pages 123-144

  9. Transfection of Protoplasts Prepared from Arabidopsis thaliana Leaves for Plant Virus Research

    • Naoi Hosoe, Takuya Keima, Yuji Fujimoto, Yuka Hagiwara-Komoda, Masayoshi Hashimoto, Kensaku Maejima et al.
    Pages 145-151

  10. CRISPR/Cas9-Mediated Editing of Genes Encoding rgs-CaM-like Proteins in Transgenic Potato Plants

    • Zhila Osmani, Shinnosuke Jin, Masafumi Mikami, Masaki Endo, Hiroki Atarashi, Kaien Fujino et al.
    Pages 153-165

  11. A Simplified Method to Engineer CRISPR/Cas9-Mediated Geminivirus Resistance in Plants

    • Zahir Ali, Syed Shan-e-Ali Zaidi, Manal Tashkandi, Magdy M. Mahfouz
    Pages 167-183

  12. Computational Workflow for Small RNA Profiling in Virus-Infected Plants

    • Livia Donaire, César Llave
    Pages 185-214

  13. A Sensitized Genetic Screen to Identify Novel Components and Regulators of the Host Antiviral RNA Interference Pathway

    • Zhongxin Guo, Xian-Bing Wang, Wan-Xiang Li, Shou-Wei Ding
    Pages 215-229

  14. Design, Synthesis, and Functional Analysis of Highly Specific Artificial Small RNAs with Antiviral Activity in Plants

    • Alberto Carbonell, José-Antonio Daròs
    Pages 231-246

  15. Resistance Breeding Through RNA Silencing of Host Factors Involved in Virus Replication

    • Masamichi Nishiguchi, Emran Md. Ali, Hui Chen, Masayuki Ishikawa, Kappei Kobayashi
    Pages 247-259

  16. Large-Scale Inoculation and Evaluation Methods for Attenuated Plant Viruses

    • Kenji Kubota, Yasuhiro Tomitaka
    Pages 261-272

  17. RNA Silencing-Mediated Apple Latent Spherical Virus Vaccine in Plants

    • Chunjiang Li, Noriko Yamagishi, Nobuyuki Yoshikawa
    Pages 273-288

  18. Back Matter

    Pages 289-292
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Keywords

About this book

This detailed book explores strategies that have been developed to combat plant virus infection. Beginning with a section on techniques for identifying and studying the virus resistance gene involved in plant innate immunity, the volume continues by delving into techniques related to novel mechanisms of plant virus resistance, methods for the analysis and practical use of RNA silencing, as well as methods for the development of plant viral vaccines. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Antiviral Resistance in Plants: Methods and Protocols serves as an ideal guide for researchers working to combat the serious threats plant virus diseases represent for agricultural production and beyond.

Editors and Affiliations

  • Faculty of Agriculture, Ehime University, Matsuyama, Japan

    Kappei Kobayashi, Masamichi Nishiguchi

Bibliographic Information

  • Book Title: Antiviral Resistance in Plants

  • Book Subtitle: Methods and Protocols

  • Editors: Kappei Kobayashi, Masamichi Nishiguchi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9635-3

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9634-6Published: 29 June 2019

  • Softcover ISBN: 978-1-4939-9637-7Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9635-3Published: 21 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 292

  • Number of Illustrations: 10 b/w illustrations, 27 illustrations in colour

  • Topics: Plant Sciences, Virology

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