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Progenitor Cells

Methods and Protocols

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2029)

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Table of contents (21 protocols)

  1. Front Matter

    Pages i-xiv
  2. Isolation and Characterization of Extracellular Vesicles from Mesenchymal Stromal Cells

    • Sally Yunsun Kim, Thanh Huyen Phan, Christina Limantoro, Bill Kalionis, Wojciech Chrzanowski
    Pages 15-23
  3. Droplet Digital PCR for Measuring Absolute Copies of Gene Transcripts in Human Islet-Derived Progenitor Cells

    • Cody-Lee Maynard, Wilson K. M. Wong, Anandwardhan A. Hardikar, Mugdha V. Joglekar
    Pages 37-48
  4. Isolation and In Vitro Culture of Human Gut Progenitor Cells

    • Jessica Bruce, Gerard E. Kaiko, Simon Keely
    Pages 49-62
  5. Isolation and Characterization of Colony-Forming Progenitor Cells from Adult Pancreas

    • Janine C. Quijano, Jacob R. Tremblay, Jeffrey Rawson, Hsun Teresa Ku
    Pages 63-80
  6. A Novel Gene Delivery Approach Using Metal Organic Frameworks in Human Islet-Derived Progenitor Cells

    • Arpita Poddar, Mugdha V. Joglekar, Anandwardhan A. Hardikar, Ravi Shukla
    Pages 81-91
  7. Encapsulation and Transplantation of Pancreatic Progenitor Cells

    • Luke Carroll, Auvro R. Mridha, Bernard E. Tuch
    Pages 93-102
  8. Differentiation of Urothelium from Mouse Embryonic Stem Cells in Chemically Defined Conditions

    • Badwi B. Boumelhem, Stuart T. Fraser, Stephen J. Assinder
    Pages 103-115
  9. Identification and Analysis of Mouse Erythroid Progenitor Cells

    • Chanukya K. Colonne, Jia Hao Yeo, Campbell V. McKenzie, Stuart T. Fraser
    Pages 125-145
  10. Single-Cell Assays Using Hematopoietic Stem and Progenitor Cells

    • Ashwini S. Hinge, Marie-Dominique Filippi
    Pages 147-160
  11. Isolation and Characterization of Cardiac Progenitor Cells

    • Parul Dixit, Rajesh G. Katare
    Pages 161-173
  12. Mitochondrial Assays Using Cardiac Stem Cells

    • Ayeshah A. Rosdah, Lea M. D. Delbridge, Shiang Y. Lim
    Pages 175-183
  13. High-Efficiency Lentiviral Gene Modification of Primary Murine Bone-Marrow Mesenchymal Stem Cells

    • Dario Gerace, Binhai Ren, Rosetta Martiniello-Wilks, Ann M. Simpson
    Pages 197-214
  14. Bone Marrow-Derived Progenitor Cells Mediate Immune Cell Regulation

    • Kisha N. Sivanathan, Patrick T. Coates
    Pages 215-234
  15. Flow Cytometry and Cell Sorting Using Hematopoietic Progenitor Cells

    • Sangeetha Vadakke-Madathil, Lalita S. Limaye, Vaijayanti P. Kale, Hina W. Chaudhry
    Pages 235-246
  16. Isolation of Epidermal Progenitor Cells from Rat Tympanic Membrane

    • Lawrence J. Liew, Allen Y. Wang, Rodney J. Dilley
    Pages 247-255

About this book

This volume explores the diversity in progenitor cell biology methods and uniquely describes techniques for isolating, generating, and characterizing progenitor cells from either tissue or embryonic stem cells. The chapters in this book cover topics such as isolating progenitor cells from sources including adipose tissue, blood, bone marrow, ear, gut, heart, pancreatic islets, and Whirton’s jelly; deriving neural, pancreatic, and urothelial precursors from the embryonic stem cells; and culturing isolated islets in vitro to generate progenitor cells via epithelial-to-mesenchymal transition. This book also delves into digital droplet PCR; flow cytometry and cell sorting; mitochondrial assays; calcium ratiometric signaling; and 2D gel electrophoresis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.



Cutting-edge and thorough, Progenitor Cells: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about this evolving and diverse field. 


Editors and Affiliations

  • NHMRC Clinical Trials Centre, Faculty of Medicine and Health, The University of Sydney, Camperdown, Australia

    Mugdha V. Joglekar, Anandwardhan A. Hardikar

Bibliographic Information

  • Book Title: Progenitor Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Mugdha V. Joglekar, Anandwardhan A. Hardikar

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9631-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9630-8Published: 05 July 2019

  • Softcover ISBN: 978-1-4939-9633-9Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9631-5Published: 04 July 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 289

  • Number of Illustrations: 11 b/w illustrations, 57 illustrations in colour

  • Topics: Stem Cells

Buy it now

Buying options

eBook USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access