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Stem Cell Mobilization

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Gerd Klein

    1. Department of Internal Medicine II, Center for Medical Research, University of Tübingen, Tübingen, Germany

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  2. Patrick Wuchter

    1. Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2017)

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Table of contents (17 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Evolution of Peripheral Blood Stem Cell Transplantation

    • Anthony D. Ho
    Pages 1-10

  3. In Vitro Dynamic Phenotyping for Testing Novel Mobilizing Agents

    • Motomu Tanaka
    Pages 11-27

  4. Colony Formation: An Assay of Hematopoietic Progenitor Cells

    • Romy Kronstein-Wiedemann, Torsten Tonn
    Pages 29-40

  5. Mobilization and Collection of Peripheral Blood Stem Cells in Adults: Focus on Timing and Benchmarking

    • Katharina Kriegsmann, Patrick Wuchter
    Pages 41-58

  6. Transmigration Assays for the Determination of Molecular Interactions Between Hematopoietic Stem Cells and Niche Cells

    • Reinhard Henschler, Rudolf Richter
    Pages 59-70

  7. Microfluidic Shear Force Assay to Determine Cell Adhesion Forces

    • Julia Hümmer, Julian Koc, Axel Rosenhahn, Cornelia Lee-Thedieck
    Pages 71-84

  8. A Microcavity Array-Based 3D Model System of the Hematopoietic Stem Cell Niche

    • Eric Gottwald, Cordula Nies, Patrick Wuchter, Rainer Saffrich, Roman Truckenmüller, Stefan Giselbrecht
    Pages 85-95

  9. Migration Assay for Leukemic Cells in a 3D Matrix Toward a Chemoattractant

    • Sabrina Zippel, Annamarija Raic, Cornelia Lee-Thedieck
    Pages 97-107

  10. Intravital Imaging of Blood Flow and HSPC Homing in Bone Marrow Microvessels

    • Jonas Stewen, Maria Gabriele Bixel
    Pages 109-121

  11. Assessing Cellular Hypoxic Status In Situ Within the Bone Marrow Microenvironment

    • Ute Suessbier, César Nombela-Arrieta
    Pages 123-134

  12. Analysis of Biomechanical Properties of Hematopoietic Stem and Progenitor Cells Using Real-Time Fluorescence and Deformability Cytometry

    • Angela Jacobi, Philipp Rosendahl, Martin Kräter, Marta Urbanska, Maik Herbig, Jochen Guck
    Pages 135-148

  13. Assessment of Proteolytic Activities in the Bone Marrow Microenvironment

    • Andreas Maurer, Gerd Klein, Nicole D. Staudt
    Pages 149-163

  14. Analysis of the Complement Cascade Activation During Mobilization of Hematopoietic Stem/Progenitor Cells

    • Anna I. Grabowska, Jakub M. Hawryluk
    Pages 165-175

  15. A Freezing Protocol for Hematopoietic Stem Cells

    • Petra Pavel, Sascha Laier
    Pages 177-192

  16. Assessment of Young and Aged Hematopoietic Stem Cell Activity by Competitive Serial Transplantation Assays

    • Yu Wei Zhang, Nina Cabezas-Wallscheid
    Pages 193-203

  17. Zebrafish Xenografts for the In Vivo Analysis of Healthy and Malignant Human Hematopoietic Cells

    • Martina Konantz, Joëlle S. Müller, Claudia Lengerke
    Pages 205-217

  18. Statistical and Mathematical Modeling of Spatiotemporal Dynamics of Stem Cells

    • Walter de Back, Thomas Zerjatke, Ingo Roeder
    Pages 219-243

  19. Back Matter

    Pages 245-248
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Keywords

About this book

This book provides detailed state-of-the-art protocols for analyzing individual aspects of stem cell mobilization at the molecular and cellular level with contributions encompassing the most recent technological developments. Specifically, the volume covers cell adhesion and migration assays in three-dimensional models, imaging techniques for the determination of blood flow or hypoxic status, innovative biomechanical studies of stem cells, and assays to determine the involvement of proteases or complement factors, as well as other key topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Stem Cell Mobilization: Methods and Protocols is an ideal guide for all laboratories in their endeavors to decipher the mechanisms of stem cell mobilization, ultimately helping stem cell donors and patients around the world.

Editors and Affiliations

  • Department of Internal Medicine II, Center for Medical Research, University of Tübingen, Tübingen, Germany

    Gerd Klein

  • Institute of Transfusion Medicine and Immunology, Medical Faculty Mannheim, Heidelberg University, Mannheim, Germany

    Patrick Wuchter

Bibliographic Information

  • Book Title: Stem Cell Mobilization

  • Book Subtitle: Methods and Protocols

  • Editors: Gerd Klein, Patrick Wuchter

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9574-5

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9573-8Published: 14 June 2019

  • Softcover ISBN: 978-1-4939-9576-9Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9574-5Published: 13 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 248

  • Number of Illustrations: 12 b/w illustrations, 54 illustrations in colour

  • Topics: Stem Cells

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