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Microbial Transposon Mutagenesis

Protocols and Applications

  • Book
  • © 2019

Overview

Editors:
  1. Steven C. Ricke

    1. Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Si Hong Park

    1. Department of Food Science and Technology, Oregon State University, Corvallis, USA

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    You can also search for this editor in PubMed   Google Scholar

  3. Morgan L. Davis

    1. Department of Food Science, University of Arkansas, Fayetteville, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2016)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
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  2. Transposon Protocols for Specific Microorganisms

    1. Front Matter

      Pages 1-1
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    2. Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium

      • Sardar Karash, Tieshan Jiang, Deepti Samarth, Reena Chandrashekar, Young Min Kwon
      Pages 3-15

    3. Application of Whole-Genome Sequencing to Transposon Mutants of Salmonella Heidelberg

      • Bryna Rackerby, Sang In Lee, Ian Moppert, Steven C. Ricke, KwangCheol C. Jeong, Si Hong Park
      Pages 17-27

    4. Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

      • Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W. Bayles
      Pages 29-37

    5. Transposon Mutagenesis in Streptococcus Species

      • Martin Nilsson, Michael Givskov, Tim Tolker-Nielsen
      Pages 39-49

    6. Implementation of Transposon Mutagenesis in Bifidobacterium

      • Lorena Ruiz, Douwe van Sinderen
      Pages 51-62

    7. Transposon Mutagenesis of Listeria monocytogenes

      • Oindrila Paul, Damayanti Chakravarty, Janet R. Donaldson
      Pages 63-71

    8. Transposon Mutagenesis of Foodborne Pathogenic Escherichia coli

      • Supraja Puttamreddy, F. Chris Minion
      Pages 73-80

    9. Methods for Transposon Mutagenesis in Proteus mirabilis

      • Philip N. Rather
      Pages 81-85

    10. Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives

      • Julie L. Stoudenmire, Michael Black, Pat M. Fidopiastis, Eric V. Stabb
      Pages 87-104

    11. Transposon Mutagenesis of Bacteroides fragilis

      • Yaligara Veeranagouda, Fasahath Husain, Hannah M. Wexler
      Pages 105-116

    12. Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis

      • John P. Bannantine, Denise K. Zinniel, Raúl G. Barletta
      Pages 117-125

  3. Transposon General Protocols and Applications

    1. Front Matter

      Pages 127-127
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    2. Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

      • Bijay K. Khajanchi, Pravin R. Kaldhone, Steven L. Foley
      Pages 129-139

    3. Construction of DNA Barcode-Tagged Salmonella Strains

      • Yichao Yang, Reena Chandrashekar, Steven C. Ricke, Young Min Kwon
      Pages 141-150

    4. Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples

      • Sarah Delaney, Richard Murphy, Fiona Walsh
      Pages 151-157

    5. Efficient Gene Deletion Method for Listeria monocytogenes

      • Hossam Abdelhamed, Attila Karsi, Mark L. Lawrence
      Pages 159-170

    6. Whole-Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools

      • Kody A. Bassett, Melanie R. Mormile, Ronald L. Frank
      Pages 171-180

  4. Back Matter

    Pages 181-183
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Keywords

About this book

This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

Editors and Affiliations

  • Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, USA

    Steven C. Ricke

  • Department of Food Science and Technology, Oregon State University, Corvallis, USA

    Si Hong Park

  • Department of Food Science, University of Arkansas, Fayetteville, USA

    Morgan L. Davis

Bibliographic Information

  • Book Title: Microbial Transposon Mutagenesis

  • Book Subtitle: Protocols and Applications

  • Editors: Steven C. Ricke, Si Hong Park, Morgan L. Davis

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9570-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9569-1Published: 14 June 2019

  • Softcover ISBN: 978-1-4939-9572-1Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9570-7Published: 13 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 183

  • Number of Illustrations: 12 b/w illustrations, 34 illustrations in colour

  • Topics: Microbial Genetics and Genomics, Microbiology

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