Microbial Transposon Mutagenesis

1. Front Matter

   Pages i-xi

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2. Transposon Protocols for Specific Microorganisms

   1. Front Matter

      Pages 1-1

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   2. Preparation of Transposon Library and Tn-Seq Amplicon Library for Salmonella Typhimurium

      • Sardar Karash, Tieshan Jiang, Deepti Samarth, Reena Chandrashekar, Young Min Kwon

      Pages 3-15

   3. Application of Whole-Genome Sequencing to Transposon Mutants of Salmonella Heidelberg

      • Bryna Rackerby, Sang In Lee, Ian Moppert, Steven C. Ricke, KwangCheol C. Jeong, Si Hong Park

      Pages 17-27

   4. Construction of a Sequence-Defined Transposon Mutant Library in Staphylococcus aureus

      • Jennifer L. Endres, Vijaya Kumar Yajjala, Paul D. Fey, Kenneth W. Bayles

      Pages 29-37

   5. Transposon Mutagenesis in Streptococcus Species

      • Martin Nilsson, Michael Givskov, Tim Tolker-Nielsen

      Pages 39-49

   6. Implementation of Transposon Mutagenesis in Bifidobacterium

      • Lorena Ruiz, Douwe van Sinderen

      Pages 51-62

   7. Transposon Mutagenesis of Listeria monocytogenes

      • Oindrila Paul, Damayanti Chakravarty, Janet R. Donaldson

      Pages 63-71

   8. Transposon Mutagenesis of Foodborne Pathogenic Escherichia coli

      • Supraja Puttamreddy, F. Chris Minion

      Pages 73-80

   9. Methods for Transposon Mutagenesis in Proteus mirabilis

      • Philip N. Rather

      Pages 81-85

   10. Mutagenesis of Vibrio fischeri and Other Marine Bacteria Using Hyperactive Mini-Tn5 Derivatives

       • Julie L. Stoudenmire, Michael Black, Pat M. Fidopiastis, Eric V. Stabb

       Pages 87-104

   11. Transposon Mutagenesis of Bacteroides fragilis

       • Yaligara Veeranagouda, Fasahath Husain, Hannah M. Wexler

       Pages 105-116

   12. Transposon Mutagenesis in Mycobacterium avium Subspecies Paratuberculosis

       • John P. Bannantine, Denise K. Zinniel, Raúl G. Barletta

       Pages 117-125

3. Transposon General Protocols and Applications

   1. Front Matter

      Pages 127-127

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   2. Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

      • Bijay K. Khajanchi, Pravin R. Kaldhone, Steven L. Foley

      Pages 129-139

   3. Construction of DNA Barcode-Tagged Salmonella Strains

      • Yichao Yang, Reena Chandrashekar, Steven C. Ricke, Young Min Kwon

      Pages 141-150

   4. Transposon-Aided Capture of Antibiotic Resistance Plasmids from Complex Samples

      • Sarah Delaney, Richard Murphy, Fiona Walsh

      Pages 151-157

   5. Efficient Gene Deletion Method for Listeria monocytogenes

      • Hossam Abdelhamed, Attila Karsi, Mark L. Lawrence

      Pages 159-170

   6. Whole-Genome Identification and Characterization of Bacterial Insertion Sequences Using Bioinformatic Tools

      • Kody A. Bassett, Melanie R. Mormile, Ronald L. Frank

      Pages 171-180

4. Back Matter

   Pages 181-183

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This book addresses cutting-edge techniques for researching transposon mutagenesis, an approach for identifying individual gene contributions to the phenotypic characteristics of a particular microorganism. The volume begins with methods for specific microorganisms and include protocols for individual microorganisms ranging from pathogens such as Salmonella to Bifidobacterium, a microorganism considered beneficial to humans and animals. The final section addresses more general protocols including plasmid transfer and bioinformatic tools as well as novel applications of transposon methodologies such as transposon-aided capture of antibiotic resistant plasmids. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Microbial Transposon Mutagenesis: Protocols and Applications serves as a valuable reference for scientists seeking to apply transposon mutagenesis to microbial genetic analyses and functionality.

• Phenotypic characteristics
• Transposon mutant libraries
• Whole genome sequencing
• Salmonella
• Microorganism genetics
• Bifidobacterium
• Bioinformatic tools

• Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, USA

  Steven C. Ricke

• Department of Food Science and Technology, Oregon State University, Corvallis, USA

  Si Hong Park

• Department of Food Science, University of Arkansas, Fayetteville, USA

  Morgan L. Davis

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