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Comparative Embryo Culture

Methods and Protocols

  • Book
  • © 2019

Overview

Editors:
  1. Jason R. Herrick

    1. Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, USA

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2006)

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Historical Note: Lessons Learned from Developing the First Semi-Defined Medium for Culture of One-Cell Rabbit Embryos to Expanding Blastocysts

    • Michael T. Kane
    Pages 1-12

  3. Culture of the Mouse Preimplantation Embryo

    • David K. Gardner, Thi T. Truong
    Pages 13-32

  4. In Vitro Culture of Rat Preimplantation Embryos

    • Yuksel Agca
    Pages 33-43

  5. Enabling Hamster Embryo Culture System: Development of Preimplantation Embryos

    • Polani B. Seshagiri, Venkatappa Vani
    Pages 45-61

  6. Culture of Preimplantation Rabbit Embryos

    • Michael T. Kane
    Pages 63-91

  7. In Vitro Maturation, Fertilization, and Culture of Pig Oocytes and Embryos

    • Bethany K. Redel, Lee D. Spate, Randall S. Prather
    Pages 93-103

  8. In Vitro Culture of Late Stage Pig Embryos in a Chemically Defined Medium, Porcine Blastocyst Medium (PBM)

    • Tomomi Mito, Hiroyoshi Hoshi
    Pages 105-113

  9. Production and Culture of the Bovine Embryo

    • Paula Tríbulo, Rocío Melissa Rivera, Martha Sofia Ortega Obando, Elizabeth Ann Jannaman, Peter J. Hansen
    Pages 115-129

  10. In Vitro Embryo Production in Sheep

    • S. Mondal, A. Mor, I. J. Reddy, S. Nandi, P. S. P. Gupta, A. Mishra
    Pages 131-140

  11. In Vitro Culture of Embryos from LOPU-Derived Goat Oocytes

    • Joanna Maria G. Souza-Fabjan, Ribrio Ivan T. P. Batista, Vicente José F. Freitas, Pascal Mermillod
    Pages 141-153

  12. Small Ruminants: Prepubertal Oocyte Donors

    • Dolors Izquierdo, María-Gracia Catalá, María-Teresa Paramio
    Pages 155-163

  13. In Vitro Production of Bison Embryos

    • Jennifer P. Barfield
    Pages 165-177

  14. In Vitro Embryo Production in Buffalo Species (Bubalus bubalis)

    • Marcello Rubessa, Lucia Boccia, Serena Di Francesco
    Pages 179-190

  15. In Vitro Culture of Deer Embryos

    • Debra K. Berg, Ampika Thongphakdee
    Pages 191-207

  16. In Vitro Culture of Camelid Embryos

    • Islam M. Saadeldin, Ayman Abdel-Aziz Swelum, Abdullah N. Alowaimer
    Pages 209-218

  17. In Vitro Culture of Embryos from Horses

    • Elaine M. Carnevale, Lisa J. Maclellan, Jo Anne E. Stokes
    Pages 219-227

  18. In Vitro Culture of Embryos from Domestic Cats

    • Jason R. Herrick
    Pages 229-246

  19. The Domestic Dog Embryo: In Vitro Fertilization, Culture, and Transfer

    • J. B. Nagashima, A. J. Travis, N. Songsasen
    Pages 247-267

  20. Culture of Mink Preimplantation Embryos

    • Jane C. Fenelon, Bruce D. Murphy
    Pages 269-277
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Keywords

About this book

This detailed volume contains embryo culture techniques that should inspire embryologists to consider comparative studies. The species included in this volume represent a broad range of taxa, whose embryos have vastly different culture requirements and developmental characteristics.  Among the species described in this volume, significant differences exist in the rates of cell division, the timing of the maternal to embryonic transition, the relative lipid content of the cytoplasm, the sensitivity of the embryo to specific environmental ions, the preferred nutrients and associated metabolic pathways used by the embryo, the timing and mechanisms of early lineage specification, the presence or absence of embryonic diapause, and the time from fertilization to implantation. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Comparative Embryo Culture: Methods and Protocols serves as an ideal aid for scientists seeking another species whose embryos have evolved a unique mechanism that could provide a valuable, comparative context for experimentation.

Editors and Affiliations

  • Department of Reproductive Sciences, Omaha’s Henry Doorly Zoo and Aquarium, Omaha, USA

    Jason R. Herrick

Bibliographic Information

  • Book Title: Comparative Embryo Culture

  • Book Subtitle: Methods and Protocols

  • Editors: Jason R. Herrick

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-4939-9566-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2019

  • Hardcover ISBN: 978-1-4939-9565-3Published: 29 June 2019

  • Softcover ISBN: 978-1-4939-9568-4Published: 14 August 2020

  • eBook ISBN: 978-1-4939-9566-0Published: 22 June 2019

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 397

  • Number of Illustrations: 23 b/w illustrations, 42 illustrations in colour

  • Topics: Embryology, Cell Culture, Animal Models

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