Phloem

1. Front Matter

   Pages i-xiv

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2. Investigating Phloem Anatomy

   1. Front Matter

      Pages 1-1

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   2. Optimal Preparation of Tissue Sections for Light-Microscopic Analysis of Phloem Anatomy

      • Marcelo Rodrigo Pace

      Pages 3-16

   3. Transmission Electron Microscopy of the Phloem with Minimal Artifacts

      • Pascal Hunziker, Alexander Schulz

      Pages 17-27

   4. Scanning Electron Microscopy of the Phloem

      • Daniel L. Mullendore

      Pages 29-35

   5. Noninvasive Investigation of Phloem Structure by 3D Synchrotron X-Ray Microtomography

      • Jussi-Petteri Suuronen, Tuula Jyske

      Pages 37-54

   6. Quantification of Leaf Phloem Anatomical Features with Microscopy

      • Jared J. Stewart, Onno Muller, Christopher M. Cohu, Barbara Demmig-Adams, William W. Adams III

      Pages 55-72

   7. Methods of Phloem Visualization: A Clear Future in Sight?

      • Elisabeth Truernit

      Pages 73-79

3. Visualizing Phloem Proteins

   1. Front Matter

      Pages 81-81

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   2. Super-Resolution Microscopy of Phloem Proteins

      • Ryan C. Stanfield, Alexander Schulz

      Pages 83-94

   3. Live-Cell Imaging of Fluorescently Tagged Phloem Proteins with Confocal Microscopy

      • Thibaud Cayla, Rozenn Le Hir, Sylvie Dinant

      Pages 95-108

   4. Immunohistochemical Localization of Proteins in the Phloem: Problems and Solutions

      • Christina Kühn

      Pages 109-122

4. Sampling Phloem Sap

   1. Front Matter

      Pages 123-123

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   2. Studying Phloem Loading with EDTA-Facilitated Phloem Exudate Collection and Analysis

      • Qiyu Xu, Yunjuan Ren, Johannes Liesche

      Pages 125-133

   3. Measurement of Inner Bark and Leaf Osmolality

      • Teemu Paljakka, Anna Lintunen, Yann Salmon, Teemu Hölttä

      Pages 135-142

5. Measurement of Phloem Transport

   1. Front Matter

      Pages 143-143

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   2. Using 13C to Quantify Phloem Transport on Tall Plants in the Field

      • Daniel Epron, Masako Dannoura, Caroline Plain

      Pages 145-151

   3. Noninvasive Determination of Phloem Transport Speed with Carbon-14 (14C)

      • Christopher Vincent, Peter E. H. Minchin, Johannes Liesche

      Pages 153-162

   4. Detecting Rapid Changes in Carbon Transport and Partitioning with Carbon-11 (11C)

      • Benjamin A. Babst, Richard Ferrieri, Michael Schueller

      Pages 163-176

   5. In Vivo Veritas: Radiotracers in Studies of Phloem Transport of Carbohydrate

      • Michael R. Thorpe, Peter E. H. Minchin

      Pages 177-194

This detailed book presents methods that enable the determination of parameters relevant to phloem research in the most efficient ways, putting plant scientists with access to adequate instrumentation in the position to answer any phloem-related question. The collection explores techniques that have been used for decades, such as tracing phloem transport with carbon isotopes, as well as recent developments, such as esculin-based assays of phloem transport and super-resolution microscopy of phloem proteins. As such, the book presents the state-of-the-art in phloem research and, at the same time, a starting point for the development of new methods that will fill the remaining gaps in the phloem researcher’s toolbox in the future. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and comprehensive, Phloem: Methods and Protocols is an ideal guide for all researchers exploring this challenging area of plant biology.

• Molecular processes
• Ecophysiology
• Plant physiology
• Theoretical modeling
• Phloem transport
• Carbon isotopes
• Plant development
• Plant signaling

• College of Life Sciences, Northwest A&F University, Yangling, China

  Johannes Liesche

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